Product nameHRP Stability Conjugate Reagent
See all ELISA buffer reagents
Tested applicationsSuitable for: ELISAmore details
Unsuitable for: IHC-Fr or IHC-P
Abcam’s HRP Stability Conjugate Reagent (ab171537) was developed specifically for Horseradish Peroxidase (HRP) conjugates in solution. ab171537 is readily incorporated into virtually any assay production protocol. Conjugates can be added directly to ab171537 and are then ready for storage and use. It is also an excellent diluent, and often decreases background and increases signal for improved signal-to-noise ratios. ab171537 prevents the loss of catalytic activity while it maintains the conformation of the antibody or protein-antigen portion of the conjugate.
Recommendations for Use of ab171537 HRP Stability Conjugate Reagent
- For optimal stability and performance, use ab171537 at 100% concentration
- Dilute your HRP conjugate to optimum working concentration in the HRP Stability Conjugate Reagent solution
- Use diluted conjugate working solution in your assay as normal
- Or, preferably, store away from direct light at room temperature and use as a stock solution for optimum assay-to-assay consistency
NOTE – When storing any conjugate/ HRP Stability Conjugate Reagent solution for an extended period of time: If a phosphate buffer is used for dilution we recommend a final concentration of no more than 5 mM buffer to avoid the possibility of initiating precipitation.
Storage instructionsStore at room temperature.
Storage bufferPreservative: 0.02% 5-Bromo-5-nitro-1,3-dioxane
Constituents: 98% Proprietary component, 0.02% 2-methyl-4-isothiazolin-3-one, 0.0024% StabilZyme® HRP Conjugate Stabilizer (Surmodics)
pH 6.2 - 6.7. Contains BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab171537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
HRP Stability Conjugate Reagent (ab171537) efficacy was verified by real-time stability studies that included independent testing of the enzyme activity and the immunological activity by ELISA.
The studies were conducted using a monoclonal IgG-HRP conjugate. Results of these analyses indicate superior performance of ab171537 compared to a common HRP conjugate diluent. The monoclonal IgG-HRP conjugate retained over 90% of its activity when stored in HRP Stability Conjugate Reagent for 24 months at room temperature.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab171537 has not yet been referenced specifically in any publications.