Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [YE361] to STAT6
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Conjugation: HRP
Product nameHRP Anti-STAT6 antibody [YE361]
See all STAT6 primary antibodies
DescriptionHRP Rabbit monoclonal [YE361] to STAT6
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human STAT6 aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P42226
- WB: NIH3T3 whole cell lysate. IHC-P: Normal human stomach tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Alexa Fluor® 488 Anti-STAT6 antibody [YE361] (ab196478)
- Alexa Fluor® 647 Anti-STAT6 antibody [YE361] (ab196928)
- Alexa Fluor® 594 Anti-STAT6 antibody [YE361] (ab207012)
- Alexa Fluor® 405 Anti-STAT6 antibody [YE361] (ab207013)
- Alexa Fluor® 555 Anti-STAT6 antibody [YE361] (ab207014)
- Alexa Fluor® 568 Anti-STAT6 antibody [YE361] (ab207015)
- Anti-STAT6 antibody [YE361] - BSA and Azide free (ab215995)
- PE Anti-STAT6 antibody [YE361] (ab223917)
- FITC Anti-STAT6 antibody [YE361] (ab223918)
- APC Anti-STAT6 antibody [YE361] (ab223919)
- Anti-STAT6 antibody [YE361] (ab32520)
Our Abpromise guarantee covers the use of ab196480 in the following tested applications.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).|
FunctionCarries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated following stimulation by IL-4 and IL-3.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
- Information by UniProt
- 12S1644 antibody
- D12S1644 antibody
- IL 4 STAT antibody
HRP Anti-STAT6 antibody [YE361] (ab196480) at 1/5000 dilution + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 94 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab196480 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
IHC image of STAT6 staining in a section of formalin-fixed paraffin-embedded normal human stomach*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196480 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
ab196480 has not yet been referenced specifically in any publications.