RecombinantRabMAb

Recombinant HRP Anti-STAT6 antibody [YE361] (ab196480)

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Western blot - HRP Anti-STAT6 antibody [YE361] (ab196480)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-STAT6 antibody [YE361] (ab196480)
  • HRP Anti-STAT6 antibody [YE361] (ab196480)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [YE361] to STAT6
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-STAT6 antibody [YE361]
    See all STAT6 primary antibodies

  • Description

    HRP Rabbit monoclonal [YE361] to STAT6

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human STAT6 aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P42226

  • Positive control

    • WB: NIH3T3 whole cell lysate. IHC-P: Normal human stomach tissue.

  • General notes

     

     

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab196480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).

Target

  • Function

    Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.

  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.

  • Post-translational
    modifications

    Tyrosine phosphorylated following stimulation by IL-4 and IL-3.

  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt

  • Database links

    • Alternative names

      • 12S1644 antibody
      • D12S1644 antibody
      • IL 4 STAT antibody
      • IL-4 Stat antibody
      • IL4 STAT antibody
      • Interleukin 4 Induced antibody
      • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
      • Signal transducer and activator of transcription 6 antibody
      • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
      • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
      • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
      • STAT 6 antibody
      • STAT interleukin4 induced antibody
      • STAT, interleukin4 induced antibody
      • Stat6 antibody
      • STAT6_HUMAN antibody
      • STAT6B antibody
      • STAT6C antibody
      • Transcription factor IL 4 STAT antibody
      see all

    Images

    • Western blot - HRP Anti-STAT6 antibody [YE361] (ab196480)
      Western blot - HRP Anti-STAT6 antibody [YE361] (ab196480)
      HRP Anti-STAT6 antibody [YE361] (ab196480) at 1/5000 dilution + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 94 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?


      Exposure time: 20 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab196480 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-STAT6 antibody [YE361] (ab196480)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-STAT6 antibody [YE361] (ab196480)

      IHC image of STAT6 staining in a section of formalin-fixed paraffin-embedded normal human stomach*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196480 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    • HRP Anti-STAT6 antibody [YE361] (ab196480)
      HRP Anti-STAT6 antibody [YE361] (ab196480)

    References (0)

    Publishing research using ab196480? Please let us know so that we can cite the reference in this datasheet.

    ab196480 has not yet been referenced specifically in any publications.

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