Recombinant HRP Anti-STING antibody [EPR13130] (ab198951)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR13130] to STING
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-STING antibody [EPR13130]
See all STING primary antibodies -
Description
HRP Rabbit monoclonal [EPR13130] to STING -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human TMEM173 aa 250 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q86WV6 -
Positive control
- WB: THP-1 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13130 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-STING antibody [EPR13130] (ab181125)
- Alexa Fluor® 488 Anti-STING antibody [EPR13130] (ab198950)
- Alexa Fluor® 647 Anti-STING antibody [EPR13130] (ab198952)
- Alexa Fluor® 594 Anti-STING antibody [EPR13130] (ab207288)
- PE Anti-STING antibody [EPR13130] (ab208874)
- Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab198951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
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Notes |
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WB
1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). |
Target
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Function
Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the TMEM173 family. -
Post-translational
modificationsPhosphorylated on tyrosine residues upon MHC-II aggregation (By similarity). Phosphorylated on Ser-358 by TBK1, leading to activation and production of IFN-beta.
Ubiquitinated. 'Lys-63'-linked ubiquitination mediated by TRIM56 at Lys-150 promotes homodimerization and recruitment of the antiviral kinase TBK1 and subsequent production of IFN-beta. 'Lys-48'-linked polyubiquitination at Lys-150 occurring after viral infection is mediated by RNF5 and leads to proteasomal degradation. -
Cellular localization
Endoplasmic reticulum membrane. Mitochondrion outer membrane. Cell membrane. Cytoplasm > perinuclear region. In response to double-stranded DNA stimulation, relocalizes to perinuclear region, where the kinase TBK1 is recruited. - Information by UniProt
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Database links
- Entrez Gene: 340061 Human
- Omim: 612374 Human
- SwissProt: Q86WV6 Human
- Unigene: 379754 Human
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Alternative names
- endoplasmic reticulum IFN stimulator antibody
- Endoplasmic reticulum interferon stimulator antibody
- ERIS antibody
see all
Images
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HRP Anti-STING antibody [EPR13130] (ab198951) at 1/5000 dilution + THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198951 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab198951 has been referenced in 1 publication.
- Wang ZC et al. Expression of STING and MIF in tumor infiltration lymphocytes as prognostic factors in patients with ESCC. Int J Clin Exp Pathol 10:10066-10074 (2017). PubMed: 31966897