Recombinant HRP Anti-Survivin antibody [EPR17358] (ab209466)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR17358] to Survivin
- Suitable for: WB
- Reacts with: Mouse, Rat
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-Survivin antibody [EPR17358]
See all Survivin primary antibodies -
Description
HRP Rabbit monoclonal [EPR17358] to Survivin -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: F9 and PC12 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17358 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab209466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
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Notes |
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WB
1/5000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). |
Target
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Function
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform. -
Tissue specificity
Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines. -
Sequence similarities
Belongs to the IAP family.
Contains 1 BIR repeat. -
Developmental stage
Expression is cell cycle-dependent and peaks at mitosis. -
Domain
The BIR repeat is necessary and sufficient for HBXIP binding. -
Post-translational
modificationsUbiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes. -
Cellular localization
Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes. - Information by UniProt
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Database links
- Entrez Gene: 11799 Mouse
- Entrez Gene: 64041 Rat
- SwissProt: O70201 Mouse
- SwissProt: Q9JHY7 Rat
- Unigene: 8552 Mouse
- Unigene: 54471 Rat
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Alternative names
- API4 antibody
- Apoptosis inhibitor 4 antibody
- Apoptosis inhibitor survivin antibody
see all
Images
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All lanes : HRP Anti-Survivin antibody [EPR17358] (ab209466) at 1/5000 dilution
Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 16 kDa
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209466 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab209466 has not yet been referenced specifically in any publications.