HRP Anti-T7 tag® antibody (ab19291)
Key features and details
- HRP Rabbit polyclonal to T7 tag®
- Suitable for: ELISA, ICC
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-T7 tag® antibody
See all T7 tag® primary antibodies -
Description
HRP Rabbit polyclonal to T7 tag® -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: ELISA, ICCmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
-
General notes
T7-TAG® is a trademark of EMD Biosciences, Inc.Molar enzyme/antibody protein ratio is 4:1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to horseradish peroxidase (HRP). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Related Products
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Applications
Our Abpromise guarantee covers the use of ab19291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | ||
| ICC |
ICC: 1/200 - 1/500.
WB: 1/1000 - 1/30000. Colorimetric detection: 1:1000 - 1:10000, Chemiluminescent detection: 1:1000 - 1:30000
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins. -
Alternative names
- 10 antibody
- Major capsid protein 10A antibody
- Major capsid protein antibody
see all
Images
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Western blot - HRP Anti-T7 tag® antibody (ab19291)All lanes : HRP Anti-T7 tag® antibody (ab19291) at 1/5000 dilution
Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg
Exposure time: 3 minutesDetected by chemiluminescence.
Protocols
References (0)
ab19291 has not yet been referenced specifically in any publications.
