HRP Anti-T7 tag® antibody (ab19291)

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Western blot - HRP Anti-T7 tag® antibody (ab19291)

    Key features and details

    • HRP Rabbit polyclonal to T7 tag®
    • Suitable for: ELISA, ICC
    • Reacts with: Species independent
    • Conjugation: HRP
    • Isotype: IgG

    Overview

    • Product name

      HRP Anti-T7 tag® antibody
      See all T7 tag® primary antibodies

    • Description

      HRP Rabbit polyclonal to T7 tag®

    • Host species

      Rabbit

    • Conjugation

      HRP

    • Tested applications

      Suitable for: ELISA, ICCmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      Synthetic peptide:

      MASMTGGQQMG

      conjugated to KLH.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • General notes

      T7-TAG® is a trademark of EMD Biosciences, Inc.

      Molar enzyme/antibody protein ratio is 4:1.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 6.8
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to horseradish peroxidase (HRP).

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab19291 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    ICC
    Notes
  • Application notes
    ELISA: 1/10000 - 1/100000.
    ICC: 1/200 - 1/500.
    WB: 1/1000 - 1/30000. Colorimetric detection: 1:1000 - 1:10000, Chemiluminescent detection: 1:1000 - 1:30000

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance

      The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.

    • Alternative names

      • 10 antibody
      • Major capsid protein 10A antibody
      • Major capsid protein antibody
      • Minor capsid protein 10B antibody
      • T7 epitope tag antibody
      see all

    Images

    • Western blot - HRP Anti-T7 tag® antibody (ab19291)
      Western blot - HRP Anti-T7 tag® antibody (ab19291)
      All lanes : HRP Anti-T7 tag® antibody (ab19291) at 1/5000 dilution

      Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
      Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
      Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg

      Exposure time: 3 minutes


      Detected by chemiluminescence.

    References (1)

    Publishing research using ab19291? Please let us know so that we can cite the reference in this datasheet.

    ab19291 has been referenced in 1 publication.

    • Pandey N  et al. Topoisomerase II SUMOylation activates a metaphase checkpoint via Haspin and Aurora B kinases. J Cell Biol 219:N/A (2020). PubMed: 31712254

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