HRP Anti-T7 tag® antibody (ab21477)

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Key features and details

  • HRP Rabbit polyclonal to T7 tag®
  • Suitable for: ELISA, WB, ICC
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-T7 tag® antibody
    See all T7 tag® primary antibodies

  • Description

    HRP Rabbit polyclonal to T7 tag®

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Specificity

    Ab21477 recognises T7 tag.

  • Tested applications

    Suitable for: ELISA, WB, ICCmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide:

    MASMTGGQQMG

    conjugated to KLH, corresponding to amino acids 1-11 of T7 tag
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    T7-TAG® is a trademark of EMD Biosciences, Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 42 kDa.
ICC Use a concentration of 0.5 - 2 µg/ml.

Target

  • Relevance

    The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.

  • Alternative names

    • 10 antibody
    • Major capsid protein 10A antibody
    • Major capsid protein antibody
    • Minor capsid protein 10B antibody
    • T7 epitope tag antibody
    see all

References (1)

Publishing research using ab21477? Please let us know so that we can cite the reference in this datasheet.

ab21477 has been referenced in 1 publication.

  • Li Y & Smolke CD Engineering biosynthesis of the anticancer alkaloid noscapine in yeast. Nat Commun 7:12137 (2016). WB . PubMed: 27378283

Customer reviews and Q&As

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Western blot abreview for Anti-T7 tag® antibody (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Treatment
Cells transfected with a construct expressing a T7-tagged protein
Specification
293T cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted Jul 23 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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