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  1. Link

    hrp-t7-tag-antibody-ab21477.pdf

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Tags & Cell Markers Epitope Tags T7 Tag
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HRP Anti-T7 tag® antibody (ab21477)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

Product price, shipping and contact information

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • HRP Rabbit polyclonal to T7 tag®
  • Suitable for: ELISA, WB, ICC
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG

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HA tag peptide (ab13835)

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Overview

  • Product name

    HRP Anti-T7 tag® antibody
    See all T7 tag® primary antibodies
  • Description

    HRP Rabbit polyclonal to T7 tag®
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Specificity

    Ab21477 recognises T7 tag.
  • Tested applications

    Suitable for: ELISA, WB, ICCmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide:

    MASMTGGQQMG

    conjugated to KLH, corresponding to amino acids 1-11 of T7 tag
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    T7-TAG® is a trademark of EMD Biosciences, Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • T7 Tag

Associated products

  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab21477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use a concentration of 0.01 - 0.1 µg/ml.
WB (1)
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 42 kDa.
ICC
Use a concentration of 0.5 - 2 µg/ml.
Notes
ELISA
Use a concentration of 0.01 - 0.1 µg/ml.
WB
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 42 kDa.
ICC
Use a concentration of 0.5 - 2 µg/ml.

Target

  • Relevance

    The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
  • Alternative names

    • 10 antibody
    • Major capsid protein 10A antibody
    • Major capsid protein antibody
    • Minor capsid protein 10B antibody
    • T7 epitope tag antibody
    see all

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab21477? Please let us know so that we can cite the reference in this datasheet.

ab21477 has been referenced in 1 publication.

  • Li Y & Smolke CD Engineering biosynthesis of the anticancer alkaloid noscapine in yeast. Nat Commun 7:12137 (2016). WB . PubMed: 27378283

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Western blot abreview for Anti-T7 tag® antibody (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Treatment
Cells transfected with a construct expressing a T7-tagged protein
Specification
293T cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Jul 23 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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