HRP Anti-T7 tag® antibody (ab21477)
Key features and details
- HRP Rabbit polyclonal to T7 tag®
- Suitable for: ELISA, WB, ICC
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-T7 tag® antibody
See all T7 tag® primary antibodies -
Description
HRP Rabbit polyclonal to T7 tag® -
Host species
Rabbit -
Conjugation
HRP -
Specificity
Ab21477 recognises T7 tag. -
Tested applications
Suitable for: ELISA, WB, ICCmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide:
MASMTGGQQMG
conjugated to KLH, corresponding to amino acids 1-11 of T7 tag -
General notes
T7-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Related Products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use a concentration of 0.01 - 0.1 µg/ml.
|
|
| WB | (1) |
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 42 kDa.
|
| ICC |
Use a concentration of 0.5 - 2 µg/ml.
|
| Notes |
|---|
|
ELISA
Use a concentration of 0.01 - 0.1 µg/ml. |
|
WB
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 42 kDa. |
|
ICC
Use a concentration of 0.5 - 2 µg/ml. |
Target
-
Relevance
The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins. -
Alternative names
- 10 antibody
- Major capsid protein 10A antibody
- Major capsid protein antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab21477 has been referenced in 1 publication.
- Li Y & Smolke CD Engineering biosynthesis of the anticancer alkaloid noscapine in yeast. Nat Commun 7:12137 (2016). WB . PubMed: 27378283