Recombinant HRP Anti-TNF Receptor II antibody [EPR1653] (ab200006)
![Western blot - HRP Anti-TNF Receptor II antibody [EPR1653] (ab200006)](https://www.abcam.com/ps/products/200/ab200006/Images/ab200006-247475-anti-tnf-receptor-ii-antibody-epr1653-hrp-western-blot.jpg "Western blot - HRP Anti-TNF Receptor II antibody [EPR1653] (ab200006)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR1653] to TNF Receptor II
- Suitable for: IHC-P, WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-TNF Receptor II antibody [EPR1653]
See all TNF Receptor II primary antibodies -
Description
HRP Rabbit monoclonal [EPR1653] to TNF Receptor II -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
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Positive control
- WB: Jurkat, MCF7, SW480 and THP1 whole cell lysates IHC-P: normal human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1653 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-TNF Receptor II antibody [EPR1653] (ab109322)
- Alexa Fluor® 488 Anti-TNF Receptor II antibody [EPR1653] (ab199821)
- Alexa Fluor® 647 Anti-TNF Receptor II antibody [EPR1653] (ab199822)
- PE Anti-TNF Receptor II antibody [EPR1653] (ab209582)
- PerCP Anti-TNF Receptor II antibody [EPR1653] (ab220515)
- Anti-TNF Receptor II antibody [EPR1653] - Low endotoxin, Azide free (ab221921)
- Anti-TNF Receptor II antibody [EPR1653] - BSA and Azide free (ab226058)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab200006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody.
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| WB | 1/5000. Detects a band of approximately 73 kDa (predicted molecular weight: 48 kDa). |
Target
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Function
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Post-translational
modificationsPhosphorylated; mainly on serine residues and with a very low level on threonine residues.
A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7133 Human
- Entrez Gene: 21938 Mouse
- Entrez Gene: 156767 Rat
- Omim: 191191 Human
- SwissProt: P20333 Human
- SwissProt: P25119 Mouse
- SwissProt: Q80WY6 Rat
- Unigene: 256278 Human
see all -
Alternative names
- CD120b antibody
- p75 antibody
- p75 TNF receptor antibody
see all
Images
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Western blot - HRP Anti-TNF Receptor II antibody [EPR1653] (ab200006)All lanes : HRP Anti-TNF Receptor II antibody [EPR1653] (ab200006) at 1/5000 dilution
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200006 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-TNF Receptor II antibody [EPR1653] (ab200006)](https://www.abcam.com/ps/products/200/ab200006/Images/ab200006-244550-anti-tnf-receptor-ii-antibody-epr1653-hrp-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-TNF Receptor II antibody [EPR1653] (ab200006)IHC image of TNF Receptor II staining in a section of formalin-fixed paraffin-embedded normal human kidney*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab200006 at 1/50 dilution for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![HRP Anti-TNF Receptor II antibody [EPR1653] (ab200006)](https://www.abcam.com/ps/products/200/ab200006/Images/ab200006-4-benefits-of-recombinant-antibodies.png)
HRP Anti-TNF Receptor II antibody [EPR1653] (ab200006)
Protocols
References (0)
ab200006 has not yet been referenced specifically in any publications.
