RecombinantRabMAb

Recombinant HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)
  • Western blot - HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)
  • HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR15581-39] to TOMM20 - Mitochondrial Marker
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker
    See all TOMM20 primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR15581-39] to TOMM20 - Mitochondrial Marker

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Human TOMM20 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q15388

  • Positive control

    • WB: HepG2, HeLa cell lysates. IHC-P: FFPE normal human colon tissue sections.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab205796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).

Target

  • Function

    Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.

  • Sequence similarities

    Belongs to the Tom20 family.

  • Cellular localization

    Mitochondrion outer membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • KIAA0016 antibody
    • MAS20 antibody
    • MGC117367 antibody
    • Mitochondrial 20 kDa outer membrane protein antibody
    • Mitochondrial import receptor subunit TOM20 homolog antibody
    • MOM19 antibody
    • Outer mitochondrial membrane receptor Tom20 antibody
    • TOM20 antibody
    • TOM20_HUMAN antibody
    • TOMM20 antibody
    • Translocase of outer mitochondrial membrane 20 homolog (yeast) antibody
    • Translocase of outer mitochondrial membrane 20 homolog type II antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)

    IHC image of TOMM20 staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins.  The section was then incubated with ab205796, 1/250 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)
    Western blot - HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)
    All lanes : HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796) at 1/5000 dilution

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 16 kDa
    Observed band size: 15 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205796 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)
    HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)

References (0)

Publishing research using ab205796? Please let us know so that we can cite the reference in this datasheet.

ab205796 has not yet been referenced specifically in any publications.

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