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Signal Transduction Protein Trafficking Vesicle Transport Regulation
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RecombinantRabMAb

Recombinant HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)

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  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)
  • Western blot - HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)
  • HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR15581-39] to TOMM20 - Mitochondrial Marker
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Conjugation: HRP

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Overview

  • Product name

    HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker
    See all TOMM20 primary antibodies
  • Description

    HRP Rabbit monoclonal [EPR15581-39] to TOMM20 - Mitochondrial Marker
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment within Human TOMM20 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q15388

  • Positive control

    • WB: HepG2, HeLa cell lysates. IHC-P: FFPE normal human colon tissue sections.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15581-39
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial Biogenesis
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

Associated products

  • Alternative Versions

    • Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)
    • Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205486)
    • Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205487)
    • Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-39] (ab214409)
    • Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (ab220822)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human TOMM20 protein (ab156966)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab205796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
IHC-P
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/5000. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).
Notes
IHC-P
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/5000. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).

Target

  • Function

    Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.
  • Sequence similarities

    Belongs to the Tom20 family.
  • Cellular localization

    Mitochondrion outer membrane.
  • Target information above from: UniProt accession Q15388 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9804 Human
    • Entrez Gene: 67952 Mouse
    • Entrez Gene: 266601 Rat
    • Omim: 601848 Human
    • SwissProt: Q15388 Human
    • SwissProt: Q9DCC8 Mouse
    • SwissProt: Q62760 Rat
    • Unigene: 533192 Human
    • Unigene: 380026 Mouse
    • Unigene: 6932 Mouse
    • Unigene: 2143 Rat
    see all
  • Alternative names

    • KIAA0016 antibody
    • MAS20 antibody
    • MGC117367 antibody
    • Mitochondrial 20 kDa outer membrane protein antibody
    • Mitochondrial import receptor subunit TOM20 homolog antibody
    • MOM19 antibody
    • Outer mitochondrial membrane receptor Tom20 antibody
    • TOM20 antibody
    • TOM20_HUMAN antibody
    • TOMM20 antibody
    • Translocase of outer mitochondrial membrane 20 homolog (yeast) antibody
    • Translocase of outer mitochondrial membrane 20 homolog type II antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)

    IHC image of TOMM20 staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins.  The section was then incubated with ab205796, 1/250 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)
    Western blot - HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)
    All lanes : HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796) at 1/5000 dilution

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 16 kDa
    Observed band size: 15 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205796 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)
    HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab205796? Please let us know so that we can cite the reference in this datasheet.

ab205796 has not yet been referenced specifically in any publications.

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