Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR15581-39] to TOMM20 - Mitochondrial Marker
- Suitable for: IHC-P, WB
- Reacts with: Human
- Conjugation: HRP
Product nameHRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker
See all TOMM20 primary antibodies
DescriptionHRP Rabbit monoclonal [EPR15581-39] to TOMM20 - Mitochondrial Marker
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment within Human TOMM20 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q15388
- WB: HepG2, HeLa cell lysates. IHC-P: FFPE normal human colon tissue sections.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)
- Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205486)
- Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205487)
- Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-39] (ab214409)
- Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (ab220822)
Our Abpromise guarantee covers the use of ab205796 in the following tested applications.
|IHC-P||1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/5000. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).|
FunctionCentral component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.
Sequence similaritiesBelongs to the Tom20 family.
Cellular localizationMitochondrion outer membrane.
- Information by UniProt
- KIAA0016 antibody
- MAS20 antibody
- MGC117367 antibody
IHC image of TOMM20 staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab205796, 1/250 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
All lanes : HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796) at 1/5000 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205796 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab205796 has not yet been referenced specifically in any publications.