HRP Anti-Transferrin antibody (ab19178)

Reviews (1)Q&A (1)

Key features and details

  • HRP Goat polyclonal to Transferrin
  • Reacts with: Human
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-Transferrin antibody
    See all Transferrin primary antibodies

  • Description

    HRP Goat polyclonal to Transferrin

  • Host species

    Goat

  • Conjugation

    HRP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Unfortunately, this information is considered to be commercially sensitive

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

  • Application notes
    ELISA: 1/10000 - 1/100000.
    ICC: 1/200 - 1/500.
    WB: 1/1000 - 1/10000 (Colorimetric detection), 1/1000 - 1/30000 (Chemiluminescent detection): Predicted molecular weight: 77 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.

    • Tissue specificity

      Expressed by the liver and secreted in plasma.

    • Involvement in disease

      Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.

    • Sequence similarities

      Belongs to the transferrin family.
      Contains 2 transferrin-like domains.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • Apotransferrin antibody
        • Beta 1 metal binding globulin antibody
        • Beta-1 metal-binding globulin antibody
        • DKFZp781D0156 antibody
        • PRO1400 antibody
        • PRO1557 antibody
        • PRO2086 antibody
        • Serotransferrin antibody
        • Serotransferrin precursor antibody
        • Siderophilin antibody
        • TF antibody
        • TFQTL1 antibody
        • Transferin antibody
        • Transferrin antibody
        • TRFE_HUMAN antibody
        see all

      References (0)

      Publishing research using ab19178? Please let us know so that we can cite the reference in this datasheet.

      ab19178 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      Do you have a WB protocol: dilution, incubation time, etc. for this antibody? The general guidelines in the website are not enough for customers not used to work with primary antibodies conjugated to HRP.

      Read More
      Answer

      Thank you for your enquiry. The recommended antibody dilutions for this antibody as detailed on the datasheet are: WB: 1/1000 - 1/10000 (Colorimetric detection), 1/1000 - 1/30000 (Chemiluminescent detection): Predicted molecular weight: 77 kDa. We do not have a recommended protocol specifically for this antibody. However, I would recommend that your customer follows our standard western blot protocol located at the following weblink omitting step (I) and applying the HRP antibody as a secondary antibody following the blocking of the membrane. https://www.abcam.com/assets/pdf/protocols/WB-beginner.pdf I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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      ELISA abreview for Anti-Transferrin antibody (HRP)

       Good
      Abreviews
      Application
      ELISA
      Sample
      Human Purified protein (serum)
      Specification
      serum
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
      Type
      Direct
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 27 2006

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