Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR2932(2)] to Transferrin - Serum Loading Control
- Suitable for: IHC-P, WB
- Reacts with: Human
- Conjugation: HRP
Product nameHRP Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control
See all Transferrin primary antibodies
DescriptionHRP Rabbit monoclonal [EPR2932(2)] to Transferrin - Serum Loading Control
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human Transferrin aa 100-200. The exact sequence is proprietary.
- Liver tissue. Fetal liver or prostate cancer lysate. IHC-P: cancerous human liver
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab185070 in the following tested applications.
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody.
|WB||1/5000. Detects a band of approximately 82 kDa (predicted molecular weight: 77 kDa).|
FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Tissue specificityExpressed by the liver and secreted in plasma.
Involvement in diseaseDefects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
Sequence similaritiesBelongs to the transferrin family.
Contains 2 transferrin-like domains.
- Information by UniProt
- Apotransferrin antibody
- Beta 1 metal binding globulin antibody
- Beta-1 metal-binding globulin antibody
HRP Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab185070) at 1/5000 dilution + Human prostate tumor tissue lysate (adenocarcinoma) - total protein (ab30305) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 77 kDa
Observed band size: 82 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab185070 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
IHC image of Transferrin staining in a section of formalin-fixed paraffin-embedded cancerous human liver*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab185070 at 10µg/ml. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ab185070 has been referenced in 1 publication.
- Seidl CI et al. CRISPR-Cas9 targeting of MMP13 in human chondrocytes leads to significantly reduced levels of the metalloproteinase and enhanced type II collagen accumulation. Osteoarthritis Cartilage 27:140-147 (2019). PubMed: 30223022