HRP Anti-Treponema pallidum antibody (ab20095)
Key features and details
- HRP Rabbit polyclonal to Treponema pallidum
- Suitable for: IHC-P, ICC/IF
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-Treponema pallidum antibody
See all Treponema pallidum primary antibodies -
Description
HRP Rabbit polyclonal to Treponema pallidum -
Host species
Rabbit -
Conjugation
HRP -
Specificity
Recognises all antigens. Non reactive in RPR assay. Has not been absorbed and may react with related microoragisms. -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Highly purified Treponema pallidum.
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General notes
Titer: >1:3,000 (indirect FA). Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
Covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed. Estimated molar HRP:IgG substitution is 2-3. >95% pure, before conjugation.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.002% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
Titer: >1:3,000 (indirect FA). Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Applications
Our Abpromise guarantee covers the use of ab20095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | 1/20 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
ICC/IF | Use at an assay dependent dilution. |
Target
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Relevance
Treponema pallidum is the causative agent of syphilis. It is a gram negative spirochete, a helical to sinusoidal bacterium with outer and cytoplasmic membranes, a thin peptidoglycan layer, and periplasmic flagella. -
Alternative names
- T. pallidum antibody
Protocols
References (0)
ab20095 has not yet been referenced specifically in any publications.