HRP Anti-Treponema pallidum antibody (ab20095)

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Key features and details

  • HRP Rabbit polyclonal to Treponema pallidum
  • Suitable for: IHC-P, ICC/IF
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-Treponema pallidum antibody
    See all Treponema pallidum primary antibodies

  • Description

    HRP Rabbit polyclonal to Treponema pallidum

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Specificity

    Recognises all antigens. Non reactive in RPR assay. Has not been absorbed and may react with related microoragisms.

  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Other species

  • Immunogen

    Highly purified Treponema pallidum.

  • General notes

    Titer: >1:3,000 (indirect FA). Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

    Covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed. Estimated molar HRP:IgG substitution is 2-3. >95% pure, before conjugation.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.002% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Primary antibody notes

    Titer: >1:3,000 (indirect FA). Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab20095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/20 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent dilution.
Notes
IHC-P
1/20 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent dilution.

Target

  • Relevance

    Treponema pallidum is the causative agent of syphilis. It is a gram negative spirochete, a helical to sinusoidal bacterium with outer and cytoplasmic membranes, a thin peptidoglycan layer, and periplasmic flagella.

  • Alternative names

    • T. pallidum antibody

References (0)

Publishing research using ab20095? Please let us know so that we can cite the reference in this datasheet.

ab20095 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

IHC-P : no staining
HIER pH 6.0 citrate
incubation 1 or 2 hours at RT

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Answer

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit ab20095que vous avez reçu ne fonctionne pas comme attendu.

Comme convenu j'ai procédé à l'envoi d'une unité de ab20923; le numéro de commande de remplacement gratuit de ce produit est *********. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

La publication dont je vous ai parléesur les différentes conditions de démasquage d'antigène est : Emoto et al., J Histochem Cytochem. 2005 Nov ;53 (11) : 1311-21 (Table 2).

N'hésitez pas à nous contacter lors d'une prochaine occasion.

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Question

How was this antibody tested? Was it tested on human tissue? Can I use the DAKO HRP detection system with this antibody?

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Answer

Thank you for your enquiry. The IHC testing was done by a customer's laboratory. The antibody was used on paraffin sections after citrate antigen retrieval. They used an amplified colorimetric detection system, but I do not have any additional information about their assay system. Yes, you can use the DAKO HRP detection system. I hope this helps you. If you need additional assistance please do not hesitate to contact us again.

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