Recombinant HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788)
![Western blot - HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788)](https://www.abcam.com/ps/products/200/ab200788/Images/ab200788-248450-WBab2007881.jpg "Western blot - HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR7315] to TRIM25/EFP
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-TRIM25/EFP antibody [EPR7315]
See all TRIM25/EFP primary antibodies -
Description
HRP Rabbit monoclonal [EPR7315] to TRIM25/EFP -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human TRIM25/EFP. The exact sequence is proprietary.
Database link: Q14258 -
Positive control
- WB: K562 whole cell lysates.
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General notes
This product was previously labelled as TRIM25
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7315 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab200788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa). |
Target
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Function
Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. -
Tissue specificity
Ubiquitous. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. -
Domain
The RING-type zinc finger is important for ISG15 E3 ligase activity and autoISGylation. AutoISGylation negatively regulates ISG15 E3 ligase activity.
The C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58. -
Post-translational
modificationsAuto-ISGylated. -
Cellular localization
Cytoplasm. Colocalized with DDX58 at cytoplasmic perinuclear bodies. - Information by UniProt
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Database links
- Entrez Gene: 7706 Human
- Entrez Gene: 217069 Mouse
- Entrez Gene: 494338 Rat
- Omim: 600453 Human
- SwissProt: Q14258 Human
- SwissProt: Q61510 Mouse
- Unigene: 528952 Human
- Unigene: 248445 Mouse
see all -
Alternative names
- E3 ubiquitin/ISG15 ligase TRIM25 antibody
- EFP antibody
- Estrogen responsive finger protein antibody
see all
Images
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Western blot - HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788)HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788) at 1/5000 dilution + K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200788 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788)](https://www.abcam.com/ps/products/200/ab200788/Images/ab200788-3-benefits-of-recombinant-antibodies.png)
HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788)
References (1)
ab200788 has been referenced in 1 publication.
- Koliopoulos MG et al. Molecular mechanism of influenza A NS1-mediated TRIM25 recognition and inhibition. Nat Commun 9:1820 (2018). PubMed: 29739942
