Recombinant HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788)
![Western blot - HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788)](https://www.abcam.com/ps/products/200/ab200788/Images/ab200788-248450-WBab2007881.jpg "Western blot - HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR7315] to TRIM25/EFP
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-TRIM25/EFP antibody [EPR7315]
See all TRIM25/EFP primary antibodies -
Description
HRP Rabbit monoclonal [EPR7315] to TRIM25/EFP -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562 whole cell lysates.
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General notes
This product was previously labelled as TRIM25
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7315 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
|
| Notes |
|---|
|
WB
1/5000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa). |
Target
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Function
Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. -
Tissue specificity
Ubiquitous. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. -
Domain
The RING-type zinc finger is important for ISG15 E3 ligase activity and autoISGylation. AutoISGylation negatively regulates ISG15 E3 ligase activity.
The C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58. -
Post-translational
modificationsAuto-ISGylated. -
Cellular localization
Cytoplasm. Colocalized with DDX58 at cytoplasmic perinuclear bodies. - Information by UniProt
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Database links
- Entrez Gene: 7706 Human
- Entrez Gene: 217069 Mouse
- Entrez Gene: 494338 Rat
- Omim: 600453 Human
- SwissProt: Q14258 Human
- SwissProt: Q61510 Mouse
- Unigene: 528952 Human
- Unigene: 248445 Mouse
see all -
Alternative names
- E3 ubiquitin/ISG15 ligase TRIM25 antibody
- EFP antibody
- Estrogen responsive finger protein antibody
see all
Images
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Western blot - HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788)HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788) at 1/5000 dilution + K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200788 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788)](https://www.abcam.com/ps/products/200/ab200788/Images/ab200788-3-benefits-of-recombinant-antibodies.png)
HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab200788 has been referenced in 1 publication.
- Koliopoulos MG et al. Molecular mechanism of influenza A NS1-mediated TRIM25 recognition and inhibition. Nat Commun 9:1820 (2018). PubMed: 29739942