HRP Anti-TSH antibody [7G12] (ab6070)

Submit a review Q&A (1)References (2)

Key features and details

  • HRP Mouse monoclonal [7G12] to TSH
  • Reacts with: Human
  • Conjugation: HRP
  • Isotype: IgG1

Overview

  • Product name

    HRP Anti-TSH antibody [7G12]
    See all TSH primary antibodies

  • Description

    HRP Mouse monoclonal [7G12] to TSH

  • Host species

    Mouse

  • Conjugation

    HRP

  • Specificity

    This antibody specifically recognizes the whole molecule of TSH. It does not cross-react with human LH, FSH, HCG.

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) from human pituitary gland.

  • General notes

    This antibody (conjugate) is ELISA paired with ab6069 (capture).

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

  • Application notes
    sELISA: Use at an assay dependent dilution.
    Can be paired for ELISA with Mouse monoclonal [11E4] to TSH (ab6069).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance

      Thyroid stimulating hormone, also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs, finds its receptors on epithelial cells in the thyroid gland, and stimulates that gland to synthesize and release thyroid hormones. TSH is a glycoprotein hormone composed of two subunits which are non covalently bound to one another. The alpha subunit of TSH is also present in two other pituitary glycoprotein hormones, follicle stimulating hormone and luteinizing hormone, and, in primates, in the placental hormone chorionic gonadotropin. Each of these hormones also has a unique beta subunit, which provides receptor specificity. In other words, TSH is composed of alpha subunit bound to the TSH beta subunit, and TSH associates only with its own receptor. Free alpha and beta subunits have essentially no biological activity.

    • Cellular localization

      Secreted

    • Database links

      • Alternative names

        • CG ALPHA antibody
        • CGA antibody
        • Chorionic gonadotropin alpha polypeptide antibody
        • FSHA antibody
        • Glycoprotein hormones alpha polypeptide antibody
        • GPHA1 antibody
        • Thyroid stimulating hormone beta subunit antibody
        • Thyrotropin beta subunit antibody
        • TSH beta antibody
        • TSHA antibody
        • TSHB antibody
        see all

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (2)

      Publishing research using ab6070? Please let us know so that we can cite the reference in this datasheet.

      ab6070 has been referenced in 2 publications.

      • Li L  et al. MicroRNA-584 Impairs Cellular Proliferation and Sensitizes Osteosarcoma Cells to Cisplatin and Taxanes by Targeting CCN2. Cancer Manag Res 12:2577-2587 (2020). PubMed: 32346311
      • Zhou Y  et al. Sulfiredoxin-1 exerts anti-apoptotic and neuroprotective effects against oxidative stress-induced injury in rat cortical astrocytes following exposure to oxygen-glucose deprivation and hydrogen peroxide. Int J Mol Med 36:43-52 (2015). WB . PubMed: 25955519

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      sELISA in mouse and rat for TSH, FSH and Luteinizing Hormone
      pairs:
      Luteinizing Hormone: ab72837 (mouse), ab72838 (mouse) - test in mouse, rat
      FSH: ab21011 (mouse, capture), ab30543 (HRP)- test in sELISA, mouse, rat
      TSH: ab6070 (HRP), ab6069 - test in mouse, rat
      send info on proteins for standard curve and secondary Abs (anti-mouse IgG) if required
      Luteinizing Hormone: none
      FSH: ab51888 (human) - test in sELISA
      TSH: ab110682 (human) - test in sELISA
      ab98799 - donkey anti-mouse IgG, preabsorbed, HRP
      ab98808 - goat anti-mouse IgG, preabsorbed, HRP

      Read More
      Answer

      Thank you for your phone call. I am sending you the following discount codes for trying the antibodies in sELISA and mouse/rat. As for the proteins, I have found the following:

      Luteinizing Hormone: none
      FSH: ab51888 (human) - test in sELISA
      TSH: ab110682 (human) - test in sELISA

      Since the FSH and TSH proteins have not yet been tested in sELISA, I am sending you also discount codes for these proteins (see below). Unfortunately, we do not carry the Luteinizing Hormone protein.

      In terms of secondary antibodies, you would only need one for the Luteinizing Hormone sELISA as none of these antibodies has an HRP label. The other antibody pairs for TSH and FSH contain an HRP-labeled primary antibody.

      The suggested secondary antibodies are:
      ab98799 - donkey anti-mouse IgG, preabsorbed, HRP (https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98799.html)
      ab98808 - goat anti-mouse IgG, preabsorbed, HRP (https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98808.html)

      Testing Discount Codes:

      DISCOUNT CODE: xxx- test in mouse, rat
      DISCOUNT CODE: xxx- test in mouse, rat
      DISCOUNT CODE: xxx- test in sELISA, mouse, rat
      DISCOUNT CODE: xxx-test in sELISA, mouse, rat
      DISCOUNT CODE: xxx- test in mouse, rat
      DISCOUNT CODE: xxx- test in mouse, rat

      DISCOUNT CODE: xxx ab51888 (human FSH protein) - test in sELISA
      DISCOUNT CODE: xxx ab110682 (human TSH protein) - test in sELISA

      Expiration date: xxx

      I am very pleased to hear you would like to accept our offer and test thes antibodies and proteins in sELISA and nouse/rat. Each code will give you 1 free PRIMARY ANTIBODY or PROTEIN before the expiration date. To redeem this offer, please submit each an Abreview for sELISA and mouse/rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

      Please also remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

      Please let me know if there is anything else I can help you with.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com