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  1. Link

    hrp-tubulin-antibody-yol134-microtubule-marker-ab196583.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Microtubules
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HRP Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab196583)

  • Datasheet
  • SDS
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Western blot - HRP Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab196583)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab196583)

Key features and details

  • HRP Rat monoclonal [YOL1/34] to Tubulin - Microtubule Marker
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Conjugation: HRP
  • Isotype: IgG2a

Overview

  • Product name

    HRP Anti-Tubulin antibody [YOL1/34] - Microtubule Marker
    See all Tubulin primary antibodies
  • Description

    HRP Rat monoclonal [YOL1/34] to Tubulin - Microtubule Marker
  • Host species

    Rat
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Saccharomyces cerevisiae, Schizosaccharomyces pombe
  • Immunogen

    Full length native protein (purified) corresponding to Saccharomyces cerevisiae Tubulin.

  • Positive control

    • WB: HeLa and NIH3T3 whole cell lysates. Rat Brain tissue lysate. IHC-P: normal human colon tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    YOL1/34
  • Isotype

    IgG2a
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195883)
    • Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884)
    • Anti-Tubulin antibody [YOL1/34] - BSA and Azide free (ab264065)
    • Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Related Products

    • ECL Substrate Kit (High Sensitivity) (ab133406)

Applications

Our Abpromise guarantee covers the use of ab196583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68363 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10376 Human
    • Entrez Gene: 203068 Human
    • Entrez Gene: 7280 Human
    • Entrez Gene: 22143 Mouse
    • Entrez Gene: 22151 Mouse
    • Entrez Gene: 22153 Mouse
    • Entrez Gene: 500929 Rat
    • Omim: 191130 Human
    • Omim: 602530 Human
    • Omim: 615101 Human
    • SwissProt: P07437 Human
    • SwissProt: P68363 Human
    • SwissProt: Q13885 Human
    • SwissProt: P05213 Mouse
    • SwissProt: Q7TMM9 Mouse
    • SwissProt: Q9D6F9 Mouse
    • SwissProt: Q6P9V9 Rat
    • Unigene: 524390 Human
    • Unigene: 636480 Human
    • Unigene: 654543 Human
    • Unigene: 392113 Mouse
    • Unigene: 422827 Mouse
    • Unigene: 7420 Mouse
    • Unigene: 99661 Rat
    see all
  • Alternative names

    • Tubulin beta 2b antibody
    • Alpha tubulin antibody
    • Alpha-tubulin ubiquitous antibody
    • beta Ib tubulin antibody
    • CDCBM5 antibody
    • CDCBM6 antibody
    • fd02b12 antibody
    • K ALPHA 1 antibody
    • M40 antibody
    • OK/SW-cl.56 antibody
    • TBA1B_HUMAN antibody
    • TUBA1B antibody
    • TUBB1 antibody
    • TUBB2 antibody
    • TUBB5 antibody
    • tubulin alpha 1b antibody
    • Tubulin alpha-1B chain antibody
    • Tubulin alpha-ubiquitous chain antibody
    • Tubulin beta 1b antibody
    • Tubulin beta 2A antibody
    • tubulin beta 2A class IIa antibody
    • Tubulin beta antibody
    • tubulin beta chain antibody
    • tubulin beta class I antibody
    • tubulin beta-1 chain antibody
    • tubulin beta-2A chain antibody
    • tubulin beta-5 chain antibody
    • Tubulin K-alpha-1 antibody
    • tubulin, alpha, ubiquitous antibody
    • tubulin, beta 2A class IIa antibody
    • tubulin, beta polypeptide 2 antibody
    • tubulin, beta polypeptide antibody
    • tubulin, beta, class IIA antibody
    • wu:fd02b12 antibody
    • zgc:55461 antibody
    see all

Images

  • Western blot - HRP Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab196583)
    Western blot - HRP Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab196583)
    All lanes : HRP Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab196583) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 2 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196583 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab196583)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab196583)

    IHC image of Tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196583 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab196583? Please let us know so that we can cite the reference in this datasheet.

    ab196583 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Candida albicans Cell lysate - whole cell (Candida albicans CAI4-URA3 HA-tagged)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    0.2 cells
    Treatment
    Cells in SD were treated with 10 mM of 3AT for 30 min before inducing with different concentration of His
    Specification
    Candida albicans CAI4-URA3 HA-tagged
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Fitz Gerald S. Silao

    Verified customer

    Submitted May 07 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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