Recombinant HRP Anti-TXNRD1 antibody [EPNCIR129] (ab203464)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-TXNRD1 antibody [EPNCIR129] (ab203464)](https://www.abcam.com/ps/products/203/ab203464/Images/ab203464-254230-IHCAP0022659.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-TXNRD1 antibody [EPNCIR129] (ab203464)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPNCIR129] to TXNRD1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Overview
-
Product name
HRP Anti-TXNRD1 antibody [EPNCIR129]
See all TXNRD1 primary antibodies -
Description
HRP Rabbit monoclonal [EPNCIR129] to TXNRD1 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Mouse TXNRD1.
Database link: Q9JMH6 -
Positive control
- WB: Mouse liver, human fetal liver and rat liver tissue lysates; NIH 3T3 and RAW264.7 whole cell lysates. IHC-P: FFPE human liver hepatocellular carcinoma tissue sections.
-
General notes
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Dolph Hatfield. View antibodies from NCI Center for Cancer Research Collaboration.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPNCIR129 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab203464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 55, 67 kDa). | |
| IHC-P | 1/125. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid. -
Tissue specificity
Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver. -
Sequence similarities
Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
Contains 1 glutaredoxin domain. -
Domain
The N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays. -
Post-translational
modificationsThe N-terminus of isoform 5 is blocked.
ISGylated. -
Cellular localization
Cytoplasm and Cytoplasm. Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 7296 Human
- Entrez Gene: 50493 Mouse
- Entrez Gene: 58819 Rat
- Omim: 601112 Human
- SwissProt: Q16881 Human
- SwissProt: Q9JMH6 Mouse
- SwissProt: O89049 Rat
- Unigene: 210155 Mouse
see all -
Alternative names
- cytoplasmic antibody
- Gene associated with retinoic and IFN-induced mortality 12 protein antibody
- Gene associated with retinoic and interferon-induced mortality 12 protein antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-TXNRD1 antibody [EPNCIR129] (ab203464)
IHC image of TXNRD1 staining in a section of formalin-fixed paraffin-embedded human liver hepatocellular carcinoma tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab203464, 1/125 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
-
![Western blot - HRP Anti-TXNRD1 antibody [EPNCIR129] (ab203464)](https://www.abcam.com/ps/products/203/ab203464/Images/ab203464-247980-WBab2034641.jpg)
Western blot - HRP Anti-TXNRD1 antibody [EPNCIR129] (ab203464)All lanes : HRP Anti-TXNRD1 antibody [EPNCIR129] (ab203464) at 1/5000 dilution
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 4 : Liver (Human) Tissue Lysate - fetal normal tissue
Lane 5 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55, 67 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab203464 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![HRP Anti-TXNRD1 antibody [EPNCIR129] (ab203464)](https://www.abcam.com/ps/products/203/ab203464/Images/ab203464-4-benefits-of-recombinant-antibodies.png)
HRP Anti-TXNRD1 antibody [EPNCIR129] (ab203464)
Protocols
References (0)
ab203464 has not yet been referenced specifically in any publications.
