Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1533Y] to Tyrosine Hydroxylase
- Suitable for: IHC-P, WB
- Reacts with: Human
- Conjugation: HRP
Product nameHRP Anti-Tyrosine Hydroxylase antibody [EP1533Y]
See all Tyrosine Hydroxylase primary antibodies
DescriptionHRP Rabbit monoclonal [EP1533Y] to Tyrosine Hydroxylase
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Tyrosine Hydroxylase aa 50-150 (phospho S70). The exact sequence is proprietary.
Database link: P07101
- WB: Human adrenal gland lysate. IHC: Human cerebellum tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab193083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody.
|WB||1/5000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).|
FunctionPlays an important role in the physiology of adrenergic neurons.
Tissue specificityMainly expressed in the brain and adrenal glands.
PathwayCatecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
Involvement in diseaseDefects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
Sequence similaritiesBelongs to the biopterin-dependent aromatic amino acid hydroxylase family.
- Information by UniProt
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
IHC image of Tyrosine Hydroxylase staining in a section of formalin-fixed paraffin-embedded normal human cerebellum tissue, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab193083 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
HRP Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab193083) at 1/5000 dilution + Human adrenal normal tissue lysate - total protein (ab29249) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 8 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab193083 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab193083 has been referenced in 2 publications.
- Fedeli D et al. In vivo and in silico studies to identify mechanisms associated with Nurr1 modulation following early life exposure to permethrin in rats. Neuroscience 340:411-423 (2017). PubMed: 27826104
- Mauriello A et al. Hypertension in kidney transplantation is associated with an early renal nerve sprouting. Nephrol Dial Transplant 32:1053-1060 (2017). PubMed: 28498963