Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR17034] to Ubiquitin (linkage-specific K27)
- Suitable for: WB
- Conjugation: HRP
Product nameHRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]
See all Ubiquitin primary antibodies
DescriptionHRP Rabbit monoclonal [EPR17034] to Ubiquitin (linkage-specific K27)
Tested applicationsSuitable for: WBmore details
Predicted to work with: Mouse, Rat, Human
Synthetic peptide within Human Ubiquitin aa 1-100 (linkage-specific K27). The exact sequence is proprietary.
Database link: P0CG48
- WB: Di-Ubiquitin/Ub2 (K27 Linked).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)
- Alexa Fluor® 488 Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237254)
- Alexa Fluor® 647 Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237255)
- Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442)
Our Abpromise guarantee covers the use of ab237256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
Cellular localizationCell Membrane, Cytoplasmic and Nuclear
- Epididymis secretory protein Li 50 antibody
- FLJ25987 antibody
- HEL S 50 antibody
This blot was produced using a 4-12% Bis-Tris gel under the MES buffer system. The gel was run at 200V for 35 mins. before being transferred onto a nitrocellulose membrane at 30V for 70 mins. The membrane was then blocked for an hour using 3% non-fat milk before being incubated with ab237256 (1/1000) overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Lane 1. Di-Ubiquitin/Ub2 (Full Length) (20ng)
Lane 2. Di-Ubiquitin/Ub2 (K11 Linked) (20ng)
Lane 3. Di-Ubiquitin/Ub2 (K27 Linked) (20ng)
Lane 4. Di-Ubiquitin/Ub2 (K29 Linked) (20ng)
Lane 5. Di-Ubiquitin/Ub2 (K33 Linked ) (20ng)
Lane 6. Di-Ubiquitin/Ub2 (K48 Linked) (20ng)
Lane 7. Di-Ubiquitin/Ub2 (K6 Linked) (20ng)
Lane 8. Di-Ubiquitin/Ub2 (K63 Linked) (20ng)
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237256 has not yet been referenced specifically in any publications.