Key features and details
- HRP Mouse monoclonal [13G12AF12BB11] to UQCRC2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Product nameHRP Anti-UQCRC2 antibody [13G12AF12BB11]
See all UQCRC2 primary antibodies
DescriptionHRP Mouse monoclonal [13G12AF12BB11] to UQCRC2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Tissue, cells or virus corresponding to Cow UQCRC2.
- WB: Human skeletal muscle tissue lysate and Ramos whole cell lysate. IHC-P: Normal human colon tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab197954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
|IHC-P||1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThis is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.
Sequence similaritiesBelongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- Complex III subunit 2 antibody
- Core protein II antibody
- Cytochrome b c1 complex subunit 2 mitochondrial antibody
All lanes : HRP Anti-UQCRC2 antibody [13G12AF12BB11] (ab197954) at 1/5000 dilution
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197954 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406
IHC image of UQCRC2 staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab197954, 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ab197954 has not yet been referenced specifically in any publications.