HRP Anti-UQCRFS1/RISP antibody [5A5] (ab198392)

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Western blot - HRP Anti-UQCRFS1/RISP antibody [5A5] (ab198392)

    Key features and details

    • HRP Mouse monoclonal [5A5] to UQCRFS1/RISP
    • Suitable for: WB
    • Reacts with: Human
    • Conjugation: HRP
    • Isotype: IgG2b

    Overview

    • Product name

      HRP Anti-UQCRFS1/RISP antibody [5A5]
      See all UQCRFS1/RISP primary antibodies

    • Description

      HRP Mouse monoclonal [5A5] to UQCRFS1/RISP

    • Host species

      Mouse

    • Conjugation

      HRP

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Cow

    • Immunogen

      Other Immunogen Type corresponding to Cow UQCRFS1/RISP. Bovine Complex III.

    • Positive control

      • WB: Human heart mitochondria lysate.

    • General notes

      Previously labelled as UQCRFS1. 

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

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    Properties

    Applications

    Our Abpromise guarantee covers the use of ab198392 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 30 kDa).

    Target

    • Function

      Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
      The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex.

    • Sequence similarities

      Contains 1 Rieske domain.

    • Cellular localization

      Mitochondrion inner membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Complex III subunit 5 antibody
      • Complex III subunit IX antibody
      • Cytochrome b c1 complex subunit Rieske mitochondrial antibody
      • Cytochrome b-c1 complex subunit 11 antibody
      • Cytochrome b-c1 complex subunit 5 antibody
      • Cytochrome b6f complex iron sulfur subunit, chloroplastic antibody
      • petC antibody
      • PGR1 antibody
      • Plastohydroquinone:plastocyanin oxidoreductase iron sulfur protein antibody
      • Proton gradient regulation protein 1 antibody
      • Rieske iron sulfur protein antibody
      • Rieske iron-sulfur protein antibody
      • RIP1 antibody
      • RIS1 antibody
      • RISP antibody
      • Ubiquinol cytochrome C reductase rieske iron sulphur antibody
      • Ubiquinol cytochrome c reductase Rieske iron-sulfur polypeptide 1 antibody
      • Ubiquinol-cytochrome c reductase 8 kDa protein antibody
      • Ubiquinol-cytochrome c reductase iron-sulfur subunit antibody
      • UCRI_HUMAN antibody
      • UQCR5 antibody
      • UQCRFS1 antibody
      see all

    Images

    • Western blot - HRP Anti-UQCRFS1/RISP antibody [5A5] (ab198392)
      Western blot - HRP Anti-UQCRFS1/RISP antibody [5A5] (ab198392)
      HRP Anti-UQCRFS1/RISP antibody [5A5] (ab198392) at 1/5000 dilution + Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 30 kDa
      Observed band size: 25 kDa
      why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198392 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    References (0)

    Publishing research using ab198392? Please let us know so that we can cite the reference in this datasheet.

    ab198392 has not yet been referenced specifically in any publications.

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