HRP Anti-UQCRFS1/RISP antibody [5A5] (ab198392)

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Western blot - HRP Anti-UQCRFS1/RISP antibody [5A5] (ab198392)

    Key features and details

    • HRP Mouse monoclonal [5A5] to UQCRFS1/RISP
    • Suitable for: WB
    • Reacts with: Human
    • Conjugation: HRP
    • Isotype: IgG2b

    Overview

    • Product name

      HRP Anti-UQCRFS1/RISP antibody [5A5]
      See all UQCRFS1/RISP primary antibodies

    • Description

      HRP Mouse monoclonal [5A5] to UQCRFS1/RISP

    • Host species

      Mouse

    • Conjugation

      HRP

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Other Immunogen Type corresponding to Cow UQCRFS1/RISP. Bovine Complex III.

    • Positive control

      • WB: Human heart mitochondria lysate.

    • General notes

      Previously labelled as UQCRFS1. 

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab198392 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Rat
    Cow
    Application Abreviews Notes
    WB
    1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 30 kDa).
    Notes
    WB
    1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 30 kDa).

    Target

    • Function

      Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
      The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex.

    • Sequence similarities

      Contains 1 Rieske domain.

    • Cellular localization

      Mitochondrion inner membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Complex III subunit 5 antibody
      • Complex III subunit IX antibody
      • Cytochrome b c1 complex subunit Rieske mitochondrial antibody
      • Cytochrome b-c1 complex subunit 11 antibody
      • Cytochrome b-c1 complex subunit 5 antibody
      • Cytochrome b6f complex iron sulfur subunit, chloroplastic antibody
      • petC antibody
      • PGR1 antibody
      • Plastohydroquinone:plastocyanin oxidoreductase iron sulfur protein antibody
      • Proton gradient regulation protein 1 antibody
      • Rieske iron sulfur protein antibody
      • Rieske iron-sulfur protein antibody
      • RIP1 antibody
      • RIS1 antibody
      • RISP antibody
      • Ubiquinol cytochrome C reductase rieske iron sulphur antibody
      • Ubiquinol cytochrome c reductase Rieske iron-sulfur polypeptide 1 antibody
      • Ubiquinol-cytochrome c reductase 8 kDa protein antibody
      • Ubiquinol-cytochrome c reductase iron-sulfur subunit antibody
      • UCRI_HUMAN antibody
      • UQCR5 antibody
      • UQCRFS1 antibody
      see all

    Images

    • Western blot - HRP Anti-UQCRFS1/RISP antibody [5A5] (ab198392)
      Western blot - HRP Anti-UQCRFS1/RISP antibody [5A5] (ab198392)
      HRP Anti-UQCRFS1/RISP antibody [5A5] (ab198392) at 1/5000 dilution + Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 30 kDa
      Observed band size: 25 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198392 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    References (0)

    Publishing research using ab198392? Please let us know so that we can cite the reference in this datasheet.

    ab198392 has not yet been referenced specifically in any publications.

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