HRP Anti-V5 tag antibody (ab1325)

Reviews (2)Q&A (2)References (4)
Western blot - HRP Anti-V5 tag antibody (ab1325)

    Key features and details

    • HRP Rabbit polyclonal to V5 tag
    • Suitable for: ICC, WB, ELISA
    • Reacts with: Species independent
    • Conjugation: HRP
    • Isotype: unknown

    Overview

    • Product name

      HRP Anti-V5 tag antibody
      See all V5 tag primary antibodies

    • Description

      HRP Rabbit polyclonal to V5 tag

    • Host species

      Rabbit

    • Conjugation

      HRP

    • Specificity

      In a direct ELISA against 1 ug of V5 peptide coupled to BSA, conjugate diluted to 1/20,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.

    • Tested applications

      Suitable for: ICC, WB, ELISAmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      Synthetic peptide: GKPIPNPLLGLDST conjugated to KLH.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      Constituents: PBS, 0.2% BSA, 0.05% CMIT/MIT based preservative
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      Antibodies were immunoaffinity purified using the peptide conjugated to a solid phase support and conjugated to horseradish peroxidase.

    • Clonality

      Polyclonal

    • Isotype

      unknown

    • Light chain type

      unknown

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab1325 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC
    Use at an assay dependent dilution.
    WB (1)
    Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.Can be blocked with V5 tag peptide (ab15829).
    ELISA (1)
    Use at an assay dependent dilution.
    Notes
    ICC
    Use at an assay dependent dilution.
    WB
    Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.Can be blocked with V5 tag peptide (ab15829).
    ELISA
    Use at an assay dependent dilution.

    Target

    • Relevance

      The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

    • Alternative names

      • GKPIPNPLLGLDST epitope tag antibody
      • GKPIPNPLLGLDST tag antibody
      • Protein Rev antibody
      • Regulator of expression of viral proteins antibody
      • rev antibody
      • V5 epitope tag antibody
      see all

    Images

    • Western blot - HRP Anti-V5 tag antibody (ab1325)
      Western blot - HRP Anti-V5 tag antibody (ab1325)
      All lanes : HRP Anti-V5 tag antibody (ab1325) at 0.2 µg/ml

      Lane 1 : E. coli whole cell lysate at 0.2 µg
      Lane 2 : E. coli whole cell lysate at 0.1 µg
      Lane 3 : E. coli whole cell lysate at 0.05 µg

      Predicted band size: 1 kDa

    References (4)

    Publishing research using ab1325? Please let us know so that we can cite the reference in this datasheet.

    ab1325 has been referenced in 4 publications.

    • Xu J  et al. Nuclear carbonic anhydrase 6B associates with PRMT5 to epigenetically promote IL-12 expression in innate response. Proc Natl Acad Sci U S A 114:8620-8625 (2017). PubMed: 28739930
    • Ni TK & Kuperwasser C Premature polyadenylation of MAGI3 produces a dominantly-acting oncogene in human breast cancer. Elife 5:N/A (2016). PubMed: 27205883
    • Gin P  et al. Chylomicronemia mutations yield new insights into interactions between lipoprotein lipase and GPIHBP1. Hum Mol Genet 21:2961-72 (2012). ELISA . PubMed: 22493000
    • Levy D  et al. Lysine methylation of the NF-?B subunit RelA by SETD6 couples activity of the histone methyltransferase GLP at chromatin to tonic repression of NF-?B signaling. Nat Immunol 12:29-36 (2011). PubMed: 21131967

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-V5 tag antibody (HRP)

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    0.1 µg
    Gel Running Conditions
    Reduced Denaturing (4~20%)
    Sample
    Escherichia coli Recombinant protein (Recombinant protein with C-terminus V5 tag)
    Specification
    Recombinant protein with C-terminus V5 tag
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 30 2014

    Question

    Would it be possible to receive item ab15828 (Anti-V5 tag antibody - ChIP Grade) as a replacement for the previously ordered antibody.

    Read More
    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab15828. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

    Read More

    Question

    Hello, I am unsure if this is the correct e-mail address to send my concerns, if it isn't could you please forward this to the correct department. I recently ordered Anti-V5 tag antibody (HRP) product number ab1325. I have tried numerous different times to use this anti-body to detect a recombinant V5 tagged protein. On each attempt I have ended up with either a very high level of non specific membrane staining which makes it impossible to determine the level of recombinant protein, or a large amount of non specific bands which also make it impossible to determine the level of recombinant protein. I've tried over night blocking in both 5% fat free milk or 5% BSA and also 1hour blocking in % fat free milk. Between 5-10, 10minute washes in 0.1% PBS-Tween. And a series of dilutions with 1:5000 used the most. I have compared your antibody to a HRP-V5 tag antibody from Invitrogen, which gives a single band at the predicted mass on the same protein samples. Unless there are some very specific conditions which are needed to use this anti-body I am unable to get it to perform in a tested application as described on the datasheet. Would you be able to give me details on either a refund, or a replacement product. Regards,

    Read More
    Answer

    Thank you for your enquiry. I am sorry to hear this vial of ab1325 is giving you trouble. It does appear that this antibody is not detecting the V5 tag as expected. If you have purchased it within the last 6 months or so I would be happy to offer you a free replacement, credit or refund. In your reply, please include the purchase order number or Abcam order reference number associated with the initial order of ab1325 so I can process your request. Thanks! I look forward to hearing from you.

    Read More

    ELISA abreview for Anti-V5 tag antibody (HRP)

     Excellent
    Abreviews
    Application
    ELISA
    Sample
    Human Recombinant protein (Recombinant V5 tagged Protein)
    Specification
    Recombinant V5 tagged Protein
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Type
    Direct
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 11 2008

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