For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    hrp-v5-tag-antibody-ab1325.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Tags & Cell Markers Epitope Tags V5 Tag
Share by email

HRP Anti-V5 tag antibody (ab1325)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (4)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - HRP Anti-V5 tag antibody (ab1325)

    Key features and details

    • HRP Rabbit polyclonal to V5 tag
    • Suitable for: ICC, WB, ELISA
    • Reacts with: Species independent
    • Conjugation: HRP
    • Isotype: unknown

    You may also be interested in

    Conjugation
    Product image
    HRP Conjugation Kit - Lightning-Link® (ab102890)
    Primary
    Product image
    HRP Anti-HA tag antibody (ab128131)
    Primary
    Product image
    Anti-mCherry antibody (ab167453)

    View more associated products

    Overview

    • Product name

      HRP Anti-V5 tag antibody
      See all V5 tag primary antibodies
    • Description

      HRP Rabbit polyclonal to V5 tag
    • Host species

      Rabbit
    • Conjugation

      HRP
    • Specificity

      In a direct ELISA against 1 ug of V5 peptide coupled to BSA, conjugate diluted to 1/20,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.
    • Tested applications

      Suitable for: ICC, WB, ELISAmore details
    • Species reactivity

      Reacts with: Species independent
    • Immunogen

      Synthetic peptide: GKPIPNPLLGLDST conjugated to KLH.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Constituents: PBS, 0.2% BSA, 0.05% CMIT/MIT based preservative
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Antibodies were immunoaffinity purified using the peptide conjugated to a solid phase support and conjugated to horseradish peroxidase.
    • Clonality

      Polyclonal
    • Isotype

      unknown
    • Light chain type

      unknown
    • Research areas

      • Tags & Cell Markers
      • Epitope Tags
      • V5 Tag

    Associated products

    • Immunizing Peptide (Blocking)

      • V5 tag peptide (ab15829)
    • Related Products

      • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
      • TMB ELISA Substrate (High Sensitivity) (ab171523)
      • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
      • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
      • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
      • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
      • 450 nm Stop Solution for TMB Substrate (ab171529)
      • 650 nm Stop Solution for TMB Substrate (ab171531)
      • Immunoassay Blocking Buffer (ab171534)
      • Immunoassay Blocking (BSA Free) (ab171535)

    Applications

    Our Abpromise guarantee covers the use of ab1325 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC Use at an assay dependent dilution.
    WB Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.Can be blocked with V5 tag peptide (ab15829).
    ELISA Use at an assay dependent dilution.

    Target

    • Relevance

      The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
    • Alternative names

      • GKPIPNPLLGLDST epitope tag antibody
      • GKPIPNPLLGLDST tag antibody
      • Protein Rev antibody
      • Regulator of expression of viral proteins antibody
      • rev antibody
      • V5 epitope tag antibody
      see all

    Images

    • Western blot - HRP Anti-V5 tag antibody (ab1325)
      Western blot - HRP Anti-V5 tag antibody (ab1325)
      All lanes : HRP Anti-V5 tag antibody (ab1325) at 0.2 µg/ml

      Lane 1 : E. coli whole cell lysate at 0.2 µg
      Lane 2 : E. coli whole cell lysate at 0.1 µg
      Lane 3 : E. coli whole cell lysate at 0.05 µg

      Predicted band size: 1 kDa

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (4)

    Publishing research using ab1325? Please let us know so that we can cite the reference in this datasheet.

    ab1325 has been referenced in 4 publications.

    • Xu J  et al. Nuclear carbonic anhydrase 6B associates with PRMT5 to epigenetically promote IL-12 expression in innate response. Proc Natl Acad Sci U S A 114:8620-8625 (2017). PubMed: 28739930
    • Ni TK & Kuperwasser C Premature polyadenylation of MAGI3 produces a dominantly-acting oncogene in human breast cancer. Elife 5:N/A (2016). PubMed: 27205883
    • Gin P  et al. Chylomicronemia mutations yield new insights into interactions between lipoprotein lipase and GPIHBP1. Hum Mol Genet 21:2961-72 (2012). ELISA . PubMed: 22493000
    • Levy D  et al. Lysine methylation of the NF-?B subunit RelA by SETD6 couples activity of the histone methyltransferase GLP at chromatin to tonic repression of NF-?B signaling. Nat Immunol 12:29-36 (2011). PubMed: 21131967

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-V5 tag antibody (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    0.1 µg
    Gel Running Conditions
    Reduced Denaturing (4~20%)
    Sample
    Escherichia coli Recombinant protein (Recombinant protein with C-terminus V5 tag)
    Specification
    Recombinant protein with C-terminus V5 tag
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 30 2014

    Question

    Would it be possible to receive item ab15828 (Anti-V5 tag antibody - ChIP Grade) as a replacement for the previously ordered antibody.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 08 2011

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab15828. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

    Read More

    Abcam Scientific Support

    Answered on Dec 08 2011

    Question

    Hello, I am unsure if this is the correct e-mail address to send my concerns, if it isn't could you please forward this to the correct department. I recently ordered Anti-V5 tag antibody (HRP) product number ab1325. I have tried numerous different times to use this anti-body to detect a recombinant V5 tagged protein. On each attempt I have ended up with either a very high level of non specific membrane staining which makes it impossible to determine the level of recombinant protein, or a large amount of non specific bands which also make it impossible to determine the level of recombinant protein. I've tried over night blocking in both 5% fat free milk or 5% BSA and also 1hour blocking in % fat free milk. Between 5-10, 10minute washes in 0.1% PBS-Tween. And a series of dilutions with 1:5000 used the most. I have compared your antibody to a HRP-V5 tag antibody from Invitrogen, which gives a single band at the predicted mass on the same protein samples. Unless there are some very specific conditions which are needed to use this anti-body I am unable to get it to perform in a tested application as described on the datasheet. Would you be able to give me details on either a refund, or a replacement product. Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 18 2011

    Answer

    Thank you for your enquiry. I am sorry to hear this vial of ab1325 is giving you trouble. It does appear that this antibody is not detecting the V5 tag as expected. If you have purchased it within the last 6 months or so I would be happy to offer you a free replacement, credit or refund. In your reply, please include the purchase order number or Abcam order reference number associated with the initial order of ab1325 so I can process your request. Thanks! I look forward to hearing from you.

    Read More

    Abcam Scientific Support

    Answered on Nov 18 2011

    ELISA abreview for Anti-V5 tag antibody (HRP)

    Excellent
    Abreviews
    Abreviews
    Application
    ELISA
    Sample
    Human Recombinant protein (Recombinant V5 tagged Protein)
    Specification
    Recombinant V5 tagged Protein
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Type
    Direct
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 11 2008

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.