Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR10852(B)] to VDAC1 / Porin - Mitochondrial Loading Control
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Product nameHRP Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control
See all VDAC1 / Porin primary antibodies
DescriptionHRP Rabbit monoclonal [EPR10852(B)] to VDAC1 / Porin - Mitochondrial Loading Control
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human VDAC1/ Porin (N terminal). The exact sequence is proprietary.
Database link: P21796
- WB: HepG2, HeLa, HEK-293 HAP1 and Jurkat, whole cell and Human Kidney, Mouse Kidney and Rat Kidney tissue lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferPreservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab185063 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).|
FunctionForms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
Tissue specificityHeart, liver and skeletal muscle.
Sequence similaritiesBelongs to the eukaryotic mitochondrial porin family.
DomainConsists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
Cellular localizationMitochondrion outer membrane. Cell membrane.
- Information by UniProt
- hVDAC1 antibody
- mVDAC5 antibody
- OMP2 antibody
All lanes : HRP Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control (ab185063) at 1/5000 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 :
HeLa whole cell lysate (ab150035)
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Kidney (Mouse) Tissue Lysate
Lane 5 : Kidney (Rat) Tissue Lysate
Lane 6 : Kidney (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab185063 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Clone EPR10852(B) has been knock-out validated in its unconjugated form. Please refer to ab154856 datasheet for experimental details.
ab185063 has not yet been referenced specifically in any publications.