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  1. Link

    hrp-vimentin-antibody-v9-cytoskeleton-marker-ab196602.pdf

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Validated using a knockout cell line

HRP Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab196602)

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Western blot - HRP Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab196602)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab196602)
  • Western blot - HRP Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab196602)

Key features and details

  • HRP Mouse monoclonal [V9] to Vimentin - Cytoskeleton Marker
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human
  • Conjugation: HRP
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated

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Overview

  • Product name

    HRP Anti-Vimentin antibody [V9] - Cytoskeleton Marker
    See all Vimentin primary antibodies
  • Description

    HRP Mouse monoclonal [V9] to Vimentin - Cytoskeleton Marker
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and MOLT4 whole cell lysates. IHC-P: normal human colon tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    V9
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class III
    • Vimentin
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Cancer
    • Tumor biomarkers
    • Other
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab195877)
    • Alexa Fluor® 647 Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab195878)
    • Anti-Vimentin antibody [V9] - BSA and Azide free (ab223871)
    • Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab8069)
  • Isotype control

    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
  • Related Products

    • ECL Substrate Kit (High Sensitivity) (ab133406)
    • Human Vimentin Profiling ELISA Kit (ab173190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab196602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 54 kDa).
Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 54 kDa).

Target

  • Function

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificity

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Involvement in disease

    Cataract 30
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Domain

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • Post-translational
    modifications

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P08670 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 280955 Cow
    • Entrez Gene: 7431 Human
    • Entrez Gene: 81818 Rat
    • Omim: 193060 Human
    • SwissProt: P48616 Cow
    • SwissProt: P08670 Human
    • SwissProt: P31000 Rat
    • Unigene: 455493 Human
    • Unigene: 691131 Human
    • Unigene: 2710 Rat
    see all
  • Form

    Vimentin is found in connective tissue and in the cytoskeleton.
  • Alternative names

    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Images

  • Western blot - HRP Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab196602)
    Western blot - HRP Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab196602)
    All lanes : HRP Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab196602) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : VIM (Vimentin) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 54 kDa


    Exposure time: 1 minute


    ab196602 was shown to specifically react with Vimentin in wild-type HAP1 cells as signal was lost in VIM (Vimentin) knockout cells. Wild-type and VIM (Vimentin) knockout samples were subjected to SDS-PAGE. Ab196602 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab196602)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab196602)

    IHC image of Vimentin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196602 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - HRP Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab196602)
    Western blot - HRP Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab196602)
    All lanes : HRP Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab196602) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 54 kDa
    Observed band size: 57 kDa why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196602 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab196602? Please let us know so that we can cite the reference in this datasheet.

ab196602 has not yet been referenced specifically in any publications.

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