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    hrp-yap1-antibody-ep1674y-ab195857.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases Src Family
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RecombinantRabMAb

Recombinant HRP Anti-YAP1 antibody [EP1674Y] (ab195857)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - HRP Anti-YAP1 antibody [EP1674Y] (ab195857)
  • HRP Anti-YAP1 antibody [EP1674Y] (ab195857)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EP1674Y] to YAP1
  • Suitable for: WB
  • Reacts with: Human
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated

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Anti-YAP1 antibody [EP1674Y] - BSA and Azide free (ab172373)
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Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free (ab172374)

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Overview

  • Product name

    HRP Anti-YAP1 antibody [EP1674Y]
    See all YAP1 primary antibodies
  • Description

    HRP Rabbit monoclonal [EP1674Y] to YAP1
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Hela whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1674Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Src Family
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Signal transduction
    • Other

Associated products

  • Alternative Versions

    • Anti-YAP1 antibody [EP1674Y] - BSA and Azide free (ab172373)
    • Anti-YAP1 antibody [EP1674Y] (ab52771)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
  • Recombinant Protein

    • Recombinant Human YAP1 protein (ab132459)
  • Related Products

    • ECL Substrate Kit (High Sensitivity) (ab133406)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab195857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000. Detects a band of approximately 72 kDa (predicted molecular weight: 65 kDa).
Notes
WB
1/5000. Detects a band of approximately 72 kDa (predicted molecular weight: 65 kDa).

Target

  • Function

    Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).
  • Tissue specificity

    Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
  • Sequence similarities

    Belongs to the YORKIE family.
    Contains 2 WW domains.
  • Post-translational
    modifications

    Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage.
  • Cellular localization

    Cytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density.
  • Target information above from: UniProt accession P46937 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10413 Human
    • Omim: 606608 Human
    • SwissProt: P46937 Human
    • Unigene: 503692 Human
    • Alternative names

      • 65 kDa Yes associated protein antibody
      • 65 kDa Yes-associated protein antibody
      • COB1 antibody
      • YAp 1 antibody
      • YAP 65 antibody
      • YAP antibody
      • YAP-1 antibody
      • YAP1 antibody
      • YAP1_HUMAN antibody
      • YAP2 antibody
      • YAP65 antibody
      • yes -associated protein delta antibody
      • Yes associated protein 1 65kDa antibody
      • Yes associated protein 1 antibody
      • Yes associated protein 2 antibody
      • yes associated protein beta antibody
      • YKI antibody
      • Yorkie homolog antibody
      see all

    Images

    • Western blot - HRP Anti-YAP1 antibody [EP1674Y] (ab195857)
      Western blot - HRP Anti-YAP1 antibody [EP1674Y] (ab195857)
      HRP Anti-YAP1 antibody [EP1674Y] (ab195857) at 1/5000 dilution + HeLa whole cell lysate (ab150035) at 10 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 65 kDa
      Observed band size: 72 kDa why is the actual band size different from the predicted?


      Exposure time: 20 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab195857 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-YAP1 antibody [EP1674Y] (ab195857)
      HRP Anti-YAP1 antibody [EP1674Y] (ab195857)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab195857? Please let us know so that we can cite the reference in this datasheet.

    ab195857 has not yet been referenced specifically in any publications.

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