Recombinant HRP Anti-YAP1 antibody [EP1674Y] (ab195857)
![Western blot - HRP Anti-YAP1 antibody [EP1674Y] (ab195857)](https://www.abcam.com/ps/products/195/ab195857/Images/ab195857-238247-anti-yap1-antibody-ep1674y-hrp-western-blot.jpg "Western blot - HRP Anti-YAP1 antibody [EP1674Y] (ab195857)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1674Y] to YAP1
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-YAP1 antibody [EP1674Y]
See all YAP1 primary antibodies -
Description
HRP Rabbit monoclonal [EP1674Y] to YAP1 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human YAP1 aa 350-450 (C terminal). The exact sequence is proprietary.
(Peptide available asab173007) -
Positive control
- WB: Hela whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1674Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab195857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 72 kDa (predicted molecular weight: 65 kDa). |
Target
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Function
Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3). -
Tissue specificity
Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level). -
Sequence similarities
Belongs to the YORKIE family.
Contains 2 WW domains. -
Post-translational
modificationsPhosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage. -
Cellular localization
Cytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density. - Information by UniProt
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Database links
- Entrez Gene: 10413 Human
- Omim: 606608 Human
- SwissProt: P46937 Human
- Unigene: 503692 Human
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Alternative names
- 65 kDa Yes associated protein antibody
- 65 kDa Yes-associated protein antibody
- COB1 antibody
see all
Images
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Western blot - HRP Anti-YAP1 antibody [EP1674Y] (ab195857)HRP Anti-YAP1 antibody [EP1674Y] (ab195857) at 1/5000 dilution +
HeLa whole cell lysate (ab150035) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab195857 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-YAP1 antibody [EP1674Y] (ab195857)](https://www.abcam.com/ps/products/195/ab195857/Images/ab195857-2-benefits-of-recombinant-antibodies.png)
HRP Anti-YAP1 antibody [EP1674Y] (ab195857)
References (0)
ab195857 has not yet been referenced specifically in any publications.
