Recombinant HRP Anti-YB1 antibody [EP2708Y] (ab204014)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-YB1 antibody [EP2708Y] (ab204014)](https://www.abcam.com/ps/products/204/ab204014/Images/ab204014-255245-IHCAP00229071.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-YB1 antibody [EP2708Y] (ab204014)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP2708Y] to YB1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-YB1 antibody [EP2708Y]
See all YB1 primary antibodies -
Description
HRP Rabbit monoclonal [EP2708Y] to YB1 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human YB1 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: A549 and MCF-7 whole cell lysates. IHC-P: FFPE human normal colon tissue sections.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2708Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab204014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 36 kDa). |
Target
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Function
Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability.
The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation. -
Sequence similarities
Contains 1 CSD (cold-shock) domain. -
Post-translational
modificationsUbiquitinated by RBBP6; leading to a decrease of YBX1 transcativational ability.
In the absence of phosphorylation the protein is retained in the cytoplasm.
Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress. - Information by UniProt
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Database links
- Entrez Gene: 4904 Human
- Entrez Gene: 22608 Mouse
- Entrez Gene: 500538 Rat
- Omim: 154030 Human
- SwissProt: P67809 Human
- SwissProt: P62960 Mouse
- SwissProt: P62961 Rat
- Unigene: 473583 Human
see all -
Alternative names
- BP 8 antibody
- CBF-A antibody
- CCAAT binding transcription factor I subunit A antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-YB1 antibody [EP2708Y] (ab204014)
IHC image of YB1 staining in a section of formalin-fixed paraffin-embedded human normal colon*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab204014 at 1/50 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - HRP Anti-YB1 antibody [EP2708Y] (ab204014)](https://www.abcam.com/ps/products/204/ab204014/Images/ab204014-254144-WBab2040141.jpg)
Western blot - HRP Anti-YB1 antibody [EP2708Y] (ab204014)All lanes : HRP Anti-YB1 antibody [EP2708Y] (ab204014) at 1/5000 dilution
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 2 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab204014 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-YB1 antibody [EP2708Y] (ab204014)](https://www.abcam.com/ps/products/204/ab204014/Images/ab204014-4-benefits-of-recombinant-antibodies.png)
HRP Anti-YB1 antibody [EP2708Y] (ab204014)
Protocols
References (0)
ab204014 has not yet been referenced specifically in any publications.
