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    hrv-3c-protease-activity-assay-kit-colorimetric-ab211088.pdf

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HRV 3C Protease Activity Assay Kit (Colorimetric) (ab211088)

  • Datasheet
  • SDS
  • Protocol Booklet
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HRV 3C Protease Activity Assay Kit (Colorimetric) (ab211088)
  • HRV 3C Protease Activity Assay Kit (Colorimetric) (ab211088)
  • HRV 3C Protease Activity Assay Kit (Colorimetric) (ab211088)

Key features and details

  • Assay type: Quantitative
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Sample type: Purified protein, Serum, Tissue Lysate, Urine
  • Sensitivity: 50 ng

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Overview

  • Product name

    HRV 3C Protease Activity Assay Kit (Colorimetric)
    See all Human Rhinovirus 3C protease kits
  • Detection method

    Colorimetric
  • Sample type

    Urine, Serum, Purified protein, Tissue Lysate
  • Assay type

    Quantitative
  • Sensitivity

    50 ng
  • Product overview

    HRV 3C Protease Activity Assay Kit (Colorimetric) (ab211088) provides a convenient method for detecting HRV 3C Protease activity in mammalian tissue samples, biological fluids and from purified proteins. The assay kit is based on the ability of a 3C Protease (derived from a HRV rhinovirus-14) to cleave a chromogenic peptide substrate to release a chromophore (pNA) which can be easily quantified using a microplate reader. This assay kit is simple, rapid and can detect HRV 3C Protease activity from as low as 50 ng protein present in samples.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K214 HRV 3C Protease Activity Assay Kit (Colorimetric). K214-100 is the same size as the 100 test size of ab211088.

    Human rhinovirus (HRV) infections are the most frequent causative agents of common cold and various other upper respiratory tract infections. Rhinoviruses are members of the picornavirus family, which have a positive-sense, single-stranded RNA genome that is translated into a single polyprotein precursor. In the case of HRVs, the viral polyprotein is mainly processed by the proteases (2A and 3C) to generate functional proteins and enzymes.

    Human rhinovirus 3C protease (EC: 3.4.22.28) is a cysteine protease that recognizes the cleavage site Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 100 tests
    HRV 3C Protease Assay Buffer 1 x 25ml
    pNA Standard (0.1 M) 1 x 20µl
    HRV 3C Protease (Positive Control) 1 x 10µl
    HRV 3C Protease Substrate 1 x 500µl
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • Other Enzymes
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • Other viruses
    • Microbiology
    • Protein
    • Viral Protein
  • Relevance

    Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro. It cleaves between Gln and Gly (independent of Pro). (HRV) 3C Protease is used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is typically dual tagged for easy removal from the sample after cleavage.

Associated products

  • Related Products

    • HRV 3C Protease Inhibitor Assay Kit (Colorimetric) (ab211089)

Images

  • HRV 3C Protease Activity Assay Kit (Colorimetric) (ab211088)
    HRV 3C Protease Activity Assay Kit (Colorimetric) (ab211088)

    Typical pNA standard calibration curve.

  • HRV 3C Protease Activity Assay Kit (Colorimetric) (ab211088)
    HRV 3C Protease Activity Assay Kit (Colorimetric) (ab211088)

    Kinetic progress curves at OD 405 nm for different amounts of HRV 3C Protease (positive control) after 1 hour incubation. Assays were performed following the kit protocol.

  • HRV 3C Protease Activity Assay Kit (Colorimetric) (ab211088)
    HRV 3C Protease Activity Assay Kit (Colorimetric) (ab211088)

    Change in OD 405 nm for different amounts of HRV 3C Protease(positive control) calculated from kinetic curves (previous figure).

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab211088? Please let us know so that we can cite the reference in this datasheet.

ab211088 has been referenced in 1 publication.

  • Meng B  et al. Inhibitory antibodies identify unique sites of therapeutic vulnerability in rhinovirus and other enteroviruses. Proc Natl Acad Sci U S A 117:13499-13508 (2020). PubMed: 32467165

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