Overview

  • Product name

    HRV 3C Protease Activity Assay Kit (Colorimetric)
    See all Human Rhinovirus 3C protease kits
  • Detection method

    Colorimetric
  • Sample type

    Urine, Serum, Purified protein, Tissue Lysate
  • Assay type

    Quantitative
  • Sensitivity

    50 ng
  • Product overview

    HRV 3C Protease Activity Assay Kit (Colorimetric) (ab211088) provides a convenient method for detecting HRV 3C Protease activity in mammalian tissue samples, biological fluids and from purified proteins. The assay kit is based on the ability of a 3C Protease (derived from a HRV rhinovirus-14) to cleave a chromogenic peptide substrate to release a chromophore (pNA) which can be easily quantified using a microplate reader. This assay kit is simple, rapid and can detect HRV 3C Protease activity from as low as 50 ng protein present in samples.

  • Notes

    Human rhinovirus (HRV) infections are the most frequent causative agents of common cold and various other upper respiratory tract infections. Rhinoviruses are members of the picornavirus family, which have a positive-sense, single-stranded RNA genome that is translated into a single polyprotein precursor. In the case of HRVs, the viral polyprotein is mainly processed by the proteases (2A and 3C) to generate functional proteins and enzymes.

    Human rhinovirus 3C protease (EC: 3.4.22.28) is a cysteine protease that recognizes the cleavage site Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.

  • Platform

    Microplate reader

Properties

Images

  • Typical pNA standard calibration curve.

  • Kinetic progress curves at OD 405 nm for different amounts of HRV 3C Protease (positive control) after 1 hour incubation. Assays were performed following the kit protocol.

  • Change in OD 405 nm for different amounts of HRV 3C Protease(positive control) calculated from kinetic curves (previous figure).

Protocols

References

ab211088 has not yet been referenced specifically in any publications.

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