Overview

  • Product name

    HRV 3C Protease Inhibitor Assay Kit (Colorimetric)
    See all Human Rhinovirus 3C protease kits
  • Detection method

    Colorimetric
  • Sample type

    Inhibitor compounds
  • Assay type

    Quantitative
  • Product overview

    HRV 3C Protease Inhibitor Screening Kit (Colorimetric) (ab211089) provides a rapid, simple, sensitive and reliable test suitable for high-throughput screening of HRV 3C Protease inhibitors. The assay is based on the ability of a 3C Protease (derived from a HRV rhinovirus-14) to cleave a chromogenic peptide substrate to release a chromophore (pNA) which can be easily quantified using a microplate reader. In the presence of a HRV 3C Protease-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the absorbance.


    This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of HRV 3C Protease.

  • Notes

    Human rhinovirus (HRV) infections are the most frequent causative agents of common cold and various other upper respiratory tract infections. Rhinoviruses are members of the picornavirus family, which have a positive-sense, single-stranded RNA genome that is translated into a single polyprotein precursor. In the case of HRVs, the viral polyprotein is mainly processed by the proteases (2A and 3C) to generate functional proteins and enzymes.

    Human rhinovirus 3C protease (EC: 3.4.22.28) is a cysteine protease that recognizes the cleavage site Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.

  • Platform

    Microplate reader

Properties

Images

  • Typical inhibition curve of HRV 3C protease activity by HRV 3C protease inhibitor (IC50 = 0.12 µM). Assay was performed following the kit protocol.

Protocols

References

ab211089 has not yet been referenced specifically in any publications.

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