Key features and details
- Rabbit polyclonal to HS1BP3 - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HS1BP3 antibody - C-terminal
DescriptionRabbit polyclonal to HS1BP3 - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
- HeLa, 293T and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab200592 was affinity purified using an epitope specific to HS1BP3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab200592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 43 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay be a modulator of IL-2 signaling.
Sequence similaritiesContains 1 PX (phox homology) domain.
- Information by UniProt
- ETM2 antibody
- FLJ14249 antibody
- H1BP3_HUMAN antibody
All lanes : Anti-HS1BP3 antibody - C-terminal (ab200592) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 43 kDa
Exposure time: 3 minutes
Detection of HS1BP3 in immunoprecipitates of HeLa whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab200592 at 6 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab200592 has not yet been referenced specifically in any publications.