Product nameHsalpha Satellite repeat ChIP primer (Reverse)
See all alpha Satellite repeat dna rna and cdna
The primer concentration is 1 micromolar (μM).
Accession number: M26919.The HsSATa primers can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells. The SATa primers are specific for a centromeric, satellite repeat on chromosome 1. In most cell types this repeat is heterochromatic and enriched with histone modifications associated with heterochromatin such as Histone H3 K9 tri methylation (see image with ab8898). Whereas it shows low levels of histone modifications associated with active gene transcription such as Histone H3 K4 tri methylation (see image with ab8580).
Tested applicationsSuitable for: Real Time PCRmore details
Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: ddH20
Concentration information loading...
RelevanceThe centromeric regions of all human chromosomes are characterized by distinct subsets of a diverse tandemly repeated DNA family, alpha satellite.
Our Abpromise guarantee covers the use of ab83621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Real Time PCR||Use at an assay dependent concentration.
Suitable for the analysis of chromatin immunoprecipitated DNA using Sybr® green real time PCR. Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product.
ab83621 has not yet been referenced specifically in any publications.