• Product name
    Anti-Hsc70 antibody [1B5]
    See all Hsc70 primary antibodies
  • Description
    Rat monoclonal [1B5] to Hsc70
  • Host species
  • Tested applications
    Suitable for: ICC/IF, IHC-P, Flow Cyt, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Monkey
  • Immunogen

    Full length native protein (purified) corresponding to Hamster Hsc70.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab51052 as a replacement.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: PBS, 50% Glycerol, 0.0017% PMSF
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab19136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 73 kDa.
IP Use a concentration of 5 µg/ml.


  • Function
    Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
  • Tissue specificity
  • Sequence similarities
    Belongs to the heat shock protein 70 family.
  • Domain
    The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410008N15Rik antibody
    • Constitutive heat shock protein 70 antibody
    • Epididymis luminal protein 33 antibody
    • Epididymis secretory sperm binding protein Li 72p antibody
    • Heat shock 70 kDa protein 8 antibody
    • Heat shock 70kD protein 10 antibody
    • Heat shock 70kD protein 8 antibody
    • Heat shock 70kDa protein 8 antibody
    • Heat shock cognate 71 kDa protein antibody
    • Heat shock cognate protein 54 antibody
    • Heat shock cognate protein 71 kDa antibody
    • Heat shock protein 8 antibody
    • Heat shock protein A8 antibody
    • Heat shock protein family A (Hsp70) member 8 antibody
    • Heat-shock70-KD protein 10, formerly antibody
    • HEL 33 antibody
    • HEL S 72p antibody
    • HSC54 antibody
    • HSC71 antibody
    • Hsc73 antibody
    • HSP71 antibody
    • HSP73 antibody
    • HSP7C_HUMAN antibody
    • HSPA10 antibody
    • HSPA8 antibody
    • LAP1 antibody
    • Lipopolysaccharide associated protein 1 antibody
    • LPS associated protein 1 antibody
    • LPS associated protein antibody
    • MGC102007 antibody
    • MGC106514 antibody
    • MGC114311 antibody
    • MGC118485 antibody
    • MGC131511 antibody
    • MGC29929 antibody
    • N-myristoyltransferase inhibitor protein 71 antibody
    • NIP71 antibody
    see all


  • All lanes : Anti-Hsc70 antibody [1B5] (ab19136) at 0.1 µg/ml

    Lane 1 : G361 human melanoma cells - whole cell lysate (50000 cells/lane)
    Lane 2 : T47D human breast cancer cells - whole cell lysate (50000 cells/lane)

    All lanes : HRP conjugated rabbit anti-rat IgG

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 73 kDa
    Observed band size: 73 kDa

    Exposure time: 15 seconds

    See Abreview

  • ab19136 staining Hsc70 in Human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/150) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rat polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Hsc70 antibody [1B5] (ab19136) at 1/20000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HEC59 whole cell lysate
    Lane 3 : HCT116 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    All lanes : Goat anti-ray HRP conjugate at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 73 kDa
    Observed band size: 70 kDa (why is the actual band size different from the predicted?)

    Exposure time: 2 seconds

    Blocking: 5% milk for 1 hour at 22°C.

    See Abreview

  • All lanes : Anti-Hsc70 antibody [1B5] (ab19136) at 0.1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Hsc70 (Hsp73) recombinant protein
    Lane 3 : Hsp70 (Hsp72) protein
    Lane 4 : Cell lysates prepared from 3T3 cells
    Lane 5 : Cell lystes prepared from human PC-12 cells
    Lane 6 : Cell lysates prepared from CHO cells
    Lane 7 : Cell lysates prepared from GPC-16 cells
    Lane 8 : Cell lysates prepared from human Hela cells

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 73 kDa

  • ab19136 at 1/150 dilution staining human HeLa cells by ICC/IF. The cells were heat shocked (2 hours at 42.5°C) and paraformaldehyde fixed prior to incubation with the antibody for 1 hour at 37°C. An Alexa-Fluor ® 568 conjugated goat anti-rat antibody was used as the secondary. The cells were also stained with DAPI to visualize the nuclei.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab19136  (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19136, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


This product has been referenced in:
  • Miles AL  et al. The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1a prolyl hydroxylation by regulating cellular iron levels. Elife 6:N/A (2017). WB ; Human . Read more (PubMed: 28296633) »
  • Messaoudi K  et al. Critical role of the HDAC6-cortactin axis in human megakaryocyte maturation leading to a proplatelet-formation defect. Nat Commun 8:1786 (2017). Read more (PubMed: 29176689) »

See all 31 Publications for this product

Customer reviews and Q&As

According to the protein database entry http://www.uniprot.org/uniprot/P11142
the protein is located as follows:

Cytoplasm. Melanosome. Nucleus › nucleolus. Note: Localized in cytoplasmic mRNP granules containing untranslated m...

Read More

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