Key features and details
- Mouse monoclonal [1F2-H5] to Hsc70
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-Hsc70 antibody [1F2-H5]
See all Hsc70 primary antibodies
DescriptionMouse monoclonal [1F2-H5] to Hsc70
SpecificityDoes not cross react with HSP70.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human Hsc70.
Database link: P11142
- WB: Mixture of 10 human cell lines; recombinant Hsc70. ICC/IF: HaCaT (human keratinocyte cell line) and HeLa (human epithelial cell line from cervix adenocarcinoma) cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab223356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionActs as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
Sequence similaritiesBelongs to the heat shock protein 70 family.
DomainThe N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 2410008N15Rik antibody
- Constitutive heat shock protein 70 antibody
- Epididymis luminal protein 33 antibody
2% formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsc70 (green) using ab223356 at a dilution of 1/100 in ICC/IF. Secondary: FITC Goat Anti-Mouse used at 1/200 dilution. The nuclear counter stain is DAPI (blue) used at 1/40,000 dilution. The right-hand panel shows the merged images.
All lanes : Anti-Hsc70 antibody [1F2-H5] (ab223356) at 1/1000 dilution
Lane 1 : Mixture of ten different human cell lines at 15 µg
Lane 2 : Recombinant Hsp72
Lane 3 : Recombinant Hsc70
All lanes : Sheep anti-mouse IgG HRP
Primary incubation: 2 hours at room temperature.
2% formaldehyde-fixed heat-shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsc70 (yellow) using ab223356 at a dilution of 1/100 in ICC/IF. Secondary: Goat Anti-Mouse at 1/200. The nuclear counter stain is DAPI at 1/40,000 (blue). The right-hand panel shows the merged images.
Methanol-fixed HaCaT (human keratinocyte cell line) cells stained for Hsc70 (green) using ab223356 at a dilution of 1/100 in ICC/IF. Secondary: FITC Goat Anti-Mouse used at 1/50 dilution.
ab223356 has not yet been referenced specifically in any publications.