Anti-Hsc70 antibody [1F2-H5] (ab223356)
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Overview
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Product name
Anti-Hsc70 antibody [1F2-H5]
See all Hsc70 primary antibodies -
Description
Mouse monoclonal [1F2-H5] to Hsc70 -
Host species
Mouse -
Specificity
Does not cross react with HSP70. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Hsc70.
Database link: P11142 -
Positive control
- WB: Mixture of 10 human cell lines; recombinant Hsc70. ICC/IF: HaCaT (human keratinocyte cell line) and HeLa (human epithelial cell line from cervix adenocarcinoma) cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
1F2-H5 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab223356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. | |
| WB | 1/1000. |
Target
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Function
Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the heat shock protein 70 family. -
Domain
The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated. -
Cellular localization
Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 3312 Human
- Omim: 600816 Human
- SwissProt: P11142 Human
- Unigene: 180414 Human
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Alternative names
- 2410008N15Rik antibody
- Constitutive heat shock protein 70 antibody
- Epididymis luminal protein 33 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)](https://www.abcam.com/ps/products/223/ab223356/Images/ab223356-283028-anti-hsc70-antibody-1f2-h5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)2% formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsc70 (green) using ab223356 at a dilution of 1/100 in ICC/IF. Secondary: FITC Goat Anti-Mouse used at 1/200 dilution. The nuclear counter stain is DAPI (blue) used at 1/40,000 dilution. The right-hand panel shows the merged images.
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![Western blot - Anti-Hsc70 antibody [1F2-H5] (ab223356)](https://www.abcam.com/ps/products/223/ab223356/Images/ab223356-283027-anti-hsc70-antibody-1f2-h5-western-blot.png)
Western blot - Anti-Hsc70 antibody [1F2-H5] (ab223356)All lanes : Anti-Hsc70 antibody [1F2-H5] (ab223356) at 1/1000 dilution
Lane 1 : Mixture of ten different human cell lines at 15 µg
Lane 2 : Recombinant Hsp72
Lane 3 : Recombinant Hsc70
Secondary
All lanes : Sheep anti-mouse IgG HRPPrimary incubation: 2 hours at room temperature.
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![Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)](https://www.abcam.com/ps/products/223/ab223356/Images/ab223356-283029-anti-hsc70-antibody-1f2-h5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)2% formaldehyde-fixed heat-shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsc70 (yellow) using ab223356 at a dilution of 1/100 in ICC/IF. Secondary: Goat Anti-Mouse at 1/200. The nuclear counter stain is DAPI at 1/40,000 (blue). The right-hand panel shows the merged images.
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![Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)](https://www.abcam.com/ps/products/223/ab223356/Images/ab223356-283030-anti-hsc70-antibody-1f2-h5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)Methanol-fixed HaCaT (human keratinocyte cell line) cells stained for Hsc70 (green) using ab223356 at a dilution of 1/100 in ICC/IF. Secondary: FITC Goat Anti-Mouse used at 1/50 dilution.
Datasheets and documents
References
ab223356 has not yet been referenced specifically in any publications.
