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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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Anti-Hsc70 antibody [1F2-H5] (ab223356)

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Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)
  • Western blot - Anti-Hsc70 antibody [1F2-H5] (ab223356)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)

Key features and details

  • Mouse monoclonal [1F2-H5] to Hsc70
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG2a

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Secondary
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Protein
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Recombinant human Hsc70 protein (ab78431)

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Overview

  • Product name

    Anti-Hsc70 antibody [1F2-H5]
    See all Hsc70 primary antibodies
  • Description

    Mouse monoclonal [1F2-H5] to Hsc70
  • Host species

    Mouse
  • Specificity

    Does not cross react with HSP70.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Hsc70.
    Database link: P11142

  • Positive control

    • WB: Mixture of 10 human cell lines; recombinant Hsc70. ICC/IF: HaCaT (human keratinocyte cell line) and HeLa (human epithelial cell line from cervix adenocarcinoma) cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    1F2-H5
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant human Hsc70 protein (ab78431)
  • Related Products

    • Recombinant human Hsc70 protein (ab78431)

Applications

Our Abpromise guarantee covers the use of ab223356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000.

Target

  • Function

    Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the heat shock protein 70 family.
  • Domain

    The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • Cellular localization

    Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P11142 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3312 Human
    • Omim: 600816 Human
    • SwissProt: P11142 Human
    • Unigene: 180414 Human
    • Alternative names

      • 2410008N15Rik antibody
      • Constitutive heat shock protein 70 antibody
      • Epididymis luminal protein 33 antibody
      • Epididymis secretory sperm binding protein Li 72p antibody
      • Heat shock 70 kDa protein 8 antibody
      • Heat shock 70kD protein 10 antibody
      • Heat shock 70kD protein 8 antibody
      • Heat shock 70kDa protein 8 antibody
      • Heat shock cognate 71 kDa protein antibody
      • Heat shock cognate protein 54 antibody
      • Heat shock cognate protein 71 kDa antibody
      • Heat shock protein 8 antibody
      • Heat shock protein A8 antibody
      • Heat shock protein family A (Hsp70) member 8 antibody
      • Heat-shock70-KD protein 10, formerly antibody
      • HEL 33 antibody
      • HEL S 72p antibody
      • HSC54 antibody
      • HSC71 antibody
      • Hsc73 antibody
      • HSP71 antibody
      • HSP73 antibody
      • HSP7C_HUMAN antibody
      • HSPA10 antibody
      • HSPA8 antibody
      • LAP1 antibody
      • Lipopolysaccharide associated protein 1 antibody
      • LPS associated protein 1 antibody
      • LPS associated protein antibody
      • MGC102007 antibody
      • MGC106514 antibody
      • MGC114311 antibody
      • MGC118485 antibody
      • MGC131511 antibody
      • MGC29929 antibody
      • N-myristoyltransferase inhibitor protein 71 antibody
      • NIP71 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)
      Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)

      2% formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsc70 (green) using ab223356 at a dilution of 1/100 in ICC/IF. Secondary: FITC Goat Anti-Mouse used at 1/200 dilution. The nuclear counter stain is DAPI (blue) used at 1/40,000 dilution. The right-hand panel shows the merged images.

    • Western blot - Anti-Hsc70 antibody [1F2-H5] (ab223356)
      Western blot - Anti-Hsc70 antibody [1F2-H5] (ab223356)
      All lanes : Anti-Hsc70 antibody [1F2-H5] (ab223356) at 1/1000 dilution

      Lane 1 : Mixture of ten different human cell lines at 15 µg
      Lane 2 : Recombinant Hsp72
      Lane 3 : Recombinant Hsc70

      Secondary
      All lanes : Sheep anti-mouse IgG HRP


      Primary incubation: 2 hours at room temperature.

    • Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)
      Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)

      2% formaldehyde-fixed heat-shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsc70 (yellow) using ab223356 at a dilution of 1/100 in ICC/IF. Secondary: Goat Anti-Mouse at 1/200. The nuclear counter stain is DAPI at 1/40,000 (blue). The right-hand panel shows the merged images.

    • Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)
      Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [1F2-H5] (ab223356)

      Methanol-fixed HaCaT (human keratinocyte cell line) cells stained for Hsc70 (green) using ab223356 at a dilution of 1/100 in ICC/IF. Secondary: FITC Goat Anti-Mouse used at 1/50 dilution.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab223356? Please let us know so that we can cite the reference in this datasheet.

    ab223356 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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