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    hsc70-antibody-ab137806.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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Anti-Hsc70 antibody (ab137806)

  • Datasheet
  • SDS
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Western blot - Anti-Hsc70 antibody (ab137806)
  • Western blot - Anti-Hsc70 antibody (ab137806)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody (ab137806)

Key features and details

  • Rabbit polyclonal to Hsc70
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant human Hsc70 protein (ab78431)

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Overview

  • Product name

    Anti-Hsc70 antibody
    See all Hsc70 primary antibodies
  • Description

    Rabbit polyclonal to Hsc70
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Zebrafish
  • Immunogen

    A recombinant fragment corresponding to a region within amino acids 139-426 of Human Hsc70 (Uniprot ID: P11142).

  • Positive control

    • A431; HeLa; Neuro2A, GL261 and C8D30 whole cell lysates; Human NCIN87 xenograft tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant human Hsc70 protein (ab78431)
  • Related Products

    • Recombinant human Hsc70 protein (ab78431)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 70 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 70 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the heat shock protein 70 family.
  • Domain

    The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • Cellular localization

    Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P11142 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 395853 Chicken
    • Entrez Gene: 281831 Cow
    • Entrez Gene: 3312 Human
    • Entrez Gene: 15481 Mouse
    • Entrez Gene: 24468 Rat
    • Entrez Gene: 573376 Zebrafish
    • Omim: 600816 Human
    • SwissProt: O73885 Chicken
    • SwissProt: P11142 Human
    • SwissProt: P63017 Mouse
    • SwissProt: P63018 Rat
    • SwissProt: Q90473 Zebrafish
    • Unigene: 180414 Human
    • Unigene: 290774 Mouse
    • Unigene: 336743 Mouse
    • Unigene: 351377 Mouse
    • Unigene: 412745 Mouse
    • Unigene: 120392 Rat
    • Unigene: 201298 Rat
    • Unigene: 75087 Zebrafish
    see all
  • Alternative names

    • 2410008N15Rik antibody
    • Constitutive heat shock protein 70 antibody
    • Epididymis luminal protein 33 antibody
    • Epididymis secretory sperm binding protein Li 72p antibody
    • Heat shock 70 kDa protein 8 antibody
    • Heat shock 70kD protein 10 antibody
    • Heat shock 70kD protein 8 antibody
    • Heat shock 70kDa protein 8 antibody
    • Heat shock cognate 71 kDa protein antibody
    • Heat shock cognate protein 54 antibody
    • Heat shock cognate protein 71 kDa antibody
    • Heat shock protein 8 antibody
    • Heat shock protein A8 antibody
    • Heat shock protein family A (Hsp70) member 8 antibody
    • Heat-shock70-KD protein 10, formerly antibody
    • HEL 33 antibody
    • HEL S 72p antibody
    • HSC54 antibody
    • HSC71 antibody
    • Hsc73 antibody
    • HSP71 antibody
    • HSP73 antibody
    • HSP7C_HUMAN antibody
    • HSPA10 antibody
    • HSPA8 antibody
    • LAP1 antibody
    • Lipopolysaccharide associated protein 1 antibody
    • LPS associated protein 1 antibody
    • LPS associated protein antibody
    • MGC102007 antibody
    • MGC106514 antibody
    • MGC114311 antibody
    • MGC118485 antibody
    • MGC131511 antibody
    • MGC29929 antibody
    • N-myristoyltransferase inhibitor protein 71 antibody
    • NIP71 antibody
    see all

Images

  • Western blot - Anti-Hsc70 antibody (ab137806)
    Western blot - Anti-Hsc70 antibody (ab137806)
    All lanes : Anti-Hsc70 antibody (ab137806) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 70 kDa



    7.5% SDS PAGE
  • Western blot - Anti-Hsc70 antibody (ab137806)
    Western blot - Anti-Hsc70 antibody (ab137806)
    All lanes : Anti-Hsc70 antibody (ab137806) at 1/1000 dilution

    Lane 1 : Neuro2A whole cell lysate
    Lane 2 : GL261 whole cell lysate
    Lane 3 : C8D30 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 70 kDa



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody (ab137806)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody (ab137806)
    Immunohistochemical analysis of paraffin-embedded Human NCIN87 xenograft tissue labelling HSc70 with ab137806 at 1/100 dilution.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab137806? Please let us know so that we can cite the reference in this datasheet.

ab137806 has been referenced in 1 publication.

  • Villamil-Ortiz JG & Cardona-Gomez GP Comparative analysis of autophagy and tauopathy related markers in cerebral ischemia and Alzheimer's disease animal models. Front Aging Neurosci 7:84 (2015). WB . PubMed: 26042033

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