Western blot abreview for Anti-Hsc70 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS and HeLa cells)
Loading amount
30 µg
Specification
U2OS and HeLa cells
Gel Running Conditions
Reduced Denaturing (12% Tris Glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/2500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in PBS

Secondary antibody

Name
Non-Abcam antibody was used: KPL
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
SuperSignal Pico
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 70 kDa

Additional data

Additional Notes
Strong Antibody. U2OS lysate on left, HeLa on right.

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Submitted Jan 18 2011

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