Western blot abreview for Anti-Hsc70 antibody

Good
Abreviews
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Sheep Serum (Seminal Plasma)
Specification
Seminal Plasma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS Tween 0.5% milk
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti Rabbit IgG (whole molecule)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Bands
Specific: 73 kDa Non-specific: 66 kDa
Exposure
2 minute(s) and 0 second(s)

Mr. Guillaume Tsikis

Verified customer

Submitted Jul 15 2013

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