Overview

  • Product name

  • Description

    Rabbit polyclonal to Hsc70
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Hsc70 aa 550-646 (C terminal).
    Database link: P11142

  • Positive control

    • IHC-P: Mouse backskin and inflamed colon tissues; Human colon carcinoma tissue. WB: Heat shocked HeLa cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab223611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 70 kDa.
IHC-P 1/100.
ICC/IF 1/250.

Target

  • Function

    Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the heat shock protein 70 family.
  • Domain

    The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • Cellular localization

    Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2410008N15Rik antibody
    • Constitutive heat shock protein 70 antibody
    • Epididymis luminal protein 33 antibody
    • Epididymis secretory sperm binding protein Li 72p antibody
    • Heat shock 70 kDa protein 8 antibody
    • Heat shock 70kD protein 10 antibody
    • Heat shock 70kD protein 8 antibody
    • Heat shock 70kDa protein 8 antibody
    • Heat shock cognate 71 kDa protein antibody
    • Heat shock cognate protein 54 antibody
    • Heat shock cognate protein 71 kDa antibody
    • Heat shock protein 8 antibody
    • Heat shock protein A8 antibody
    • Heat shock protein family A (Hsp70) member 8 antibody
    • Heat-shock70-KD protein 10, formerly antibody
    • HEL 33 antibody
    • HEL S 72p antibody
    • HSC54 antibody
    • HSC71 antibody
    • Hsc73 antibody
    • HSP71 antibody
    • HSP73 antibody
    • HSP7C_HUMAN antibody
    • HSPA10 antibody
    • HSPA8 antibody
    • LAP1 antibody
    • Lipopolysaccharide associated protein 1 antibody
    • LPS associated protein 1 antibody
    • LPS associated protein antibody
    • MGC102007 antibody
    • MGC106514 antibody
    • MGC114311 antibody
    • MGC118485 antibody
    • MGC131511 antibody
    • MGC29929 antibody
    • N-myristoyltransferase inhibitor protein 71 antibody
    • NIP71 antibody
    see all

Images

  • Mouse backskin tissue stained for Hsc70 using ab223611 at 1/100 dilution for 1 hour at room temperature in immunohistochemical analysis, followed by Goat Anti-Rabbit FITC (green) at 1/50 for 1 hour at room temperature. Fixation: Bouin’s Fixative Solution.

  • Anti-Hsc70 antibody (ab223611) at 1/1000 dilution + heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysates at 15 µg

    Secondary
    Donkey Anti-Rabbit IgG HRP

    Predicted band size: 70 kDa



    Blocking buffer: 1.5% BSA for 30 minutes at room temperature.

  • Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Hsc70 using ab223611 at 1/10000 dilution for 12 hours at 4°C in immunohistochemical analysis, followed by Goat Anti-Rabbit Biotin at 1/2000 for 1 hour at room temperature. Counterstain: Methyl Green for 2 minutes at room temperature.

  • Formalin-fixed, paraffin-embedded mouse inflamed colon tissue stained for Hsc70 using ab223611 at 1/50000 dilution for 12 hours at 4°C in immunohistochemical analysis, followed by Goat Anti-Rabbit Biotin at 1/2000 for 1 hour at room temperature. Counterstain: Methyl Green for 2 minutes at room temperature.

References

ab223611 has not yet been referenced specifically in any publications.

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