Key features and details
- Rabbit polyclonal to Hsc70
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Hsc70 antibody
See all Hsc70 primary antibodies
DescriptionRabbit polyclonal to Hsc70
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Hamster, Cow, Monkey
Synthetic peptide corresponding to Human Hsc70. Synthetic peptide surrounding amino acid 559.
Database link: P11142
- IHC-P: human brain cortex. WB: Jurkat cell lysate; mouse small intestine lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol, 0.5% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab230770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 70 kDa.|
FunctionActs as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
Sequence similaritiesBelongs to the heat shock protein 70 family.
DomainThe N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 2410008N15Rik antibody
- Constitutive heat shock protein 70 antibody
- Epididymis luminal protein 33 antibody
Formalin-fixed, paraffin-embedded human brain cortex tissue stained for Hsc70 using ab230770 at 5 ug/ml in immunohistochemical analysis.
All lanes : Anti-Hsc70 antibody (ab230770) at 4 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : Mouse small intestine lysate
Predicted band size: 70 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230770 has not yet been referenced specifically in any publications.