Anti-Hsc70 antibody [EP1531Y] (ab51052)
- Datasheet
- Specific References (12)
- Protocols
Overview
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Product nameAnti-Hsc70 antibody [EP1531Y]
See all Hsc70 primary antibodies -
DescriptionRabbit monoclonal [EP1531Y] to Hsc70
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Host speciesRabbit
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Tested applicationsSuitable for: Flow Cyt, IP, WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human Hsc70 aa 600 to the C-terminus (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HeLa cell lysate IHC-P: Human breast carcinoma tissue ICC/IF: HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEP1531Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab51052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/30 - 1/70. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IP | 1/10 - 1/20. | |
| WB | 1/500 - 1/5000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa). | |
| IHC-P | 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/250. |
Target
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FunctionActs as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
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Tissue specificityUbiquitous.
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Sequence similaritiesBelongs to the heat shock protein 70 family.
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DomainThe N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
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Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated. -
Cellular localizationCytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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Database links
- Entrez Gene: 3312 Human
- Entrez Gene: 15481 Mouse
- Entrez Gene: 24468 Rat
- Omim: 600816 Human
- SwissProt: P11142 Human
- SwissProt: P63017 Mouse
- SwissProt: P63018 Rat
- Unigene: 180414 Human
see all -
Alternative names
- 2410008N15Rik antibody
- Constitutive heat shock protein 70 antibody
- Epididymis luminal protein 33 antibody
see all
Images
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![Western blot - Anti-Hsc70 antibody [EP1531Y] (ab51052)](https://www.abcam.com/ps/products/51/ab51052/Images/ab51052-254452-ab51052WB2.jpg)
Western blot - Anti-Hsc70 antibody [EP1531Y] (ab51052)All lanes : Anti-Hsc70 antibody [EP1531Y] (ab51052) at 1/5000 dilution (purified)
Lane 1 : C6 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : mouse heart lysate
Lane 4 : rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution
Predicted band size: 71 kDa
Observed band size: 71 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Hsc70 antibody [EP1531Y] (ab51052)](https://www.abcam.com/ps/products/51/ab51052/Images/ab51052-254451-ab51052WB1.jpg)
Western blot - Anti-Hsc70 antibody [EP1531Y] (ab51052)All lanes : Anti-Hsc70 antibody [EP1531Y] (ab51052) at 1/5000 dilution (purified)
Lane 1 : human fetal brain lysate
Lane 2 : HeLa cell lysate
Lane 3 : HEK293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution
Predicted band size: 71 kDa
Observed band size: 71 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [EP1531Y] (ab51052)](https://www.abcam.com/ps/products/51/ab51052/Images/ab51052-5-ab51052ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [EP1531Y] (ab51052)Immunofluorescence staining of MCF7 cells with purified ab51052 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab51052 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody [EP1531Y] (ab51052)](https://www.abcam.com/ps/products/51/ab51052/Images/ab51052-4-ab51052IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody [EP1531Y] (ab51052)Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab51052 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Immunoprecipitation - Anti-Hsc70 antibody [EP1531Y] (ab51052)](https://www.abcam.com/ps/products/51/ab51052/Images/ab51052-7-ab51052IP1.jpg)
Immunoprecipitation - Anti-Hsc70 antibody [EP1531Y] (ab51052)ab51052 (purified) at 1/20 immunoprecipitating Hsc70 in 10 μg HeLa (Lanes 1 and 2, observed at 71 kDa). Lane 3 - PBS. For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST -
![Flow Cytometry - Anti-Hsc70 antibody [EP1531Y] (ab51052)](https://www.abcam.com/ps/products/51/ab51052/Images/ab51052-6-ab51052FC1.jpg)
Flow Cytometry - Anti-Hsc70 antibody [EP1531Y] (ab51052)Overlay histogram showing MCF7 cells fixed in 4% PFA and stained with purified ab51052 at a dilution of 1 in 70 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line). -
![Western blot - Anti-Hsc70 antibody [EP1531Y] (ab51052)](https://www.abcam.com/ps/products/51/ab51052/Images/ab51052_1.bmp)
Western blot - Anti-Hsc70 antibody [EP1531Y] (ab51052)Anti-Hsc70 antibody [EP1531Y] (ab51052) at 1/500 dilution (unpurified) + HeLa cell lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 71 kDa
Observed band size: 71 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody [EP1531Y] (ab51052)](https://www.abcam.com/ps/products/51/ab51052/Images/ab51052_2.bmp)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody [EP1531Y] (ab51052)Unpurified ab51052 (1/250) staining Hsc70 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
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![Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [EP1531Y] (ab51052)](https://www.abcam.com/ps/products/51/ab51052/Images/ab51052_3.bmp)
Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody [EP1531Y] (ab51052)Immunofluorescent staining of HeLa cells using unpurified ab51052 (1/100).
References
This product has been referenced in:
- Magalhaes J et al. Effects of ambroxol on the autophagy-lysosome pathway and mitochondria in primary cortical neurons. Sci Rep 8:1385 (2018). WB ; Mouse . Read more (PubMed: 29362387) »
- Kitamura A et al. Different aggregation states of a nuclear localization signal-tagged 25-kDa C-terminal fragment of TAR RNA/DNA-binding protein 43 kDa. Genes Cells 22:521-534 (2017). WB . Read more (PubMed: 28497562) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"