Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1531Y] to Hsc70 (HRP)
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Product nameAnti-Hsc70 antibody [EP1531Y] (HRP)
See all Hsc70 primary antibodies
DescriptionRabbit monoclonal [EP1531Y] to Hsc70 (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Hsc70 aa 600 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P11142
- WB: HeLa whole cell lysate
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab200757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 71 kDa).|
FunctionActs as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
Sequence similaritiesBelongs to the heat shock protein 70 family.
DomainThe N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 2410008N15Rik antibody
- Constitutive heat shock protein 70 antibody
- Epididymis luminal protein 33 antibody
Anti-Hsc70 antibody [EP1531Y] (HRP) (ab200757) at 1/5000 dilution + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200757 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab200757 has not yet been referenced specifically in any publications.