Overview

  • Product name

  • Description

    Rabbit polyclonal to HSD11B1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Dog, Pig, Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    DDIHHVRKSM EVNFL

    , corresponding to internal sequence amino acids 131-145 of Human HSD11B1.

  • Positive control

    • HeLa cell lysate, SMMC cell lysate, Raji cell lysate, Colo320 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab109554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.
IHC-P Use a concentration of 1 µg/ml.
IHC-Fr Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.

Target

  • Function

    Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol.
  • Tissue specificity

    Widely expressed. Highest expression in liver.
  • Involvement in disease

    Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
  • Sequence similarities

    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 11 beta HSD 1 antibody
    • 11 beta HSD1 antibody
    • 11 beta hydroxysteroid dehydrogenase 1 antibody
    • 11 DH antibody
    • 11-beta hydroxysteroid dehydrogenase, type 1 antibody
    • 11-beta-HSD1 antibody
    • 11-beta-hydroxysteroid dehydrogenase 1 antibody
    • 11-DH antibody
    • 11DH antibody
    • Corticosteroid 11 beta dehydrogenase isozyme 1 antibody
    • Corticosteroid 11-beta-dehydrogenase isozyme 1 antibody
    • CORTRD2 antibody
    • DHI1_HUMAN antibody
    • HDL antibody
    • HSD 11 antibody
    • HSD11 antibody
    • HSD11B antibody
    • HSD11B1 antibody
    • HSD11L antibody
    • Hydroxysteroid (11 beta) dehydrogenase antibody
    • Hydroxysteroid (11 beta) dehydrogenase 1 antibody
    • MGC13539 antibody
    • SDR26C1 antibody
    • short chain dehydrogenase/reductase family 26C, member 1 antibody
    see all

Images

  • All lanes : Anti-HSD11B1 antibody (ab109554) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : SMMC whole cell lysate
    Lane 3 : Raji whole cell lysate
    Lane 4 : Colo320 whole cell lysate

    Predicted band size: 32 kDa

References

This product has been referenced in:

  • Hu Y  et al. Corticosterone-Induced Lipogenesis Activation and Lipophagy Inhibition in Chicken Liver Are Alleviated by Maternal Betaine Supplementation. J Nutr 148:316-325 (2018). Read more (PubMed: 29546310) »
  • Pathak NM  et al. Beneficial metabolic effects of dietary epigallocatechin gallate alone and in combination with exendin-4 in high fat diabetic mice. Mol Cell Endocrinol 460:200-208 (2018). Read more (PubMed: 28754350) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HK2 cell (human kidney proximal tubule))
Loading amount
30 µg
Specification
HK2 cell (human kidney proximal tubule)
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C

Mr. Dongil Kim

Verified customer

Submitted May 03 2013

Question
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry for any inconvenience that this product has caused. As requested, I have issued a free of charge replacement with the order number *******. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Read More

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1108638 with ab39364. The item was shipped yesterday and you should receive it today.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up