Product nameAnti-HSD11B1 antibody
See all HSD11B1 primary antibodies
DescriptionRabbit polyclonal to HSD11B1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
- Fetal liver lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab83522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.Can be blocked with Human HSD11B1 peptide (ab99223). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionCatalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol.
Tissue specificityWidely expressed. Highest expression in liver.
Involvement in diseaseDefects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- 11 beta HSD 1 antibody
- 11 beta HSD1 antibody
- 11 beta hydroxysteroid dehydrogenase 1 antibody
Anti-HSD11B1 antibody (ab83522) at 1 µg/ml (in 5% skim milk/ PBS) + Human fetal liver lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 32 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
All lanes : Anti-HSD11B1 antibody (ab83522) at 1/2000 dilution (in PBS + 0.1% Tween + 2.5% milk for 90 minutes at 22°C)
Lane 1 : Tissue lysate of Human liver microsomes
Lane 2 : Tissue lysate of Mouse liver microsomes
Lane 3 : Tissue lysate of Pig liver microsomes
Lysates/proteins at 25 µg per lane.
All lanes : An HRP-conjugated Donkey anti-rabbit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: ~38 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
Blocking Step : 5% Milk for 16 hours at 4°C
ab83522 at 5 µg/ml staining HSD11B1 in Human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
ICC/IF image of ab83522 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83522, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Kong S et al. Multi-transgenic minipig models exhibiting potential for hepatic insulin resistance and pancreatic apoptosis. Mol Med Rep 13:669-80 (2016). WB . Read more (PubMed: 26648014) »
- Chowdhury S et al. Decreased 11ß-Hydroxysteroid Dehydrogenase 1 Level and Activity in Murine Pancreatic Islets Caused by Insulin-Like Growth Factor I Overexpression. PLoS One 10:e0136656 (2015). Read more (PubMed: 26305481) »