Recombinant
RabMAb

Anti-HSD11B1 antibody [EP9406(2)] (ab169785)

Overview

  • Product name
    Anti-HSD11B1 antibody [EP9406(2)]
    See all HSD11B1 primary antibodies
  • Description
    Rabbit monoclonal [EP9406(2)] to HSD11B1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Guinea pig, Hamster
  • Immunogen

    Synthetic peptide, corresponding to residues in Human HSD11B1 (Uniprot ID: P28845)

  • Positive control
    • Human fetal liver lysate. Raji lysate. Human kidney and liver tissues.
  • General notes

    Mouse and rat species are recommended based on WB results, we do not guarantee IHC-P for mouse and rat.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 32 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

We do not guarantee IHC-P for mouse and rat.

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol.
    • Tissue specificity
      Widely expressed. Highest expression in liver.
    • Involvement in disease
      Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
    • Sequence similarities
      Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    • Post-translational
      modifications
      Glycosylated.
    • Cellular localization
      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 11 beta HSD 1 antibody
      • 11 beta HSD1 antibody
      • 11 beta hydroxysteroid dehydrogenase 1 antibody
      • 11 DH antibody
      • 11-beta hydroxysteroid dehydrogenase, type 1 antibody
      • 11-beta-HSD1 antibody
      • 11-beta-hydroxysteroid dehydrogenase 1 antibody
      • 11-DH antibody
      • 11DH antibody
      • Corticosteroid 11 beta dehydrogenase isozyme 1 antibody
      • Corticosteroid 11-beta-dehydrogenase isozyme 1 antibody
      • CORTRD2 antibody
      • DHI1_HUMAN antibody
      • HDL antibody
      • HSD 11 antibody
      • HSD11 antibody
      • HSD11B antibody
      • HSD11B1 antibody
      • HSD11L antibody
      • Hydroxysteroid (11 beta) dehydrogenase antibody
      • Hydroxysteroid (11 beta) dehydrogenase 1 antibody
      • MGC13539 antibody
      • SDR26C1 antibody
      • short chain dehydrogenase/reductase family 26C, member 1 antibody
      see all

    Images

    • All lanes : Anti-HSD11B1 antibody [EP9406(2)] (ab169785) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Raji lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 32 kDa

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling HSD11B1 using ab169785 at a 1/100 dilution.
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling HSD11B1 using ab169785 at a 1/100 dilution.

    References

    This product has been referenced in:
    • Li Z  et al. Hormonal changes and folliculogenesis in female offspring of rats exposed to cadmium during gestation and lactation. Environ Pollut 238:336-347 (2018). WB ; Rat . Read more (PubMed: 29574358) »
    • Rocamora-Reverte L  et al. T-cell autonomous death induced by regeneration of inert glucocorticoid metabolites. Cell Death Dis 8:e2948 (2017). Flow Cyt ; Mouse . Read more (PubMed: 28726773) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Flow Cytometry
    Sample
    Mouse Cell (murine T cells from spleen)
    Permeabilization
    Yes - Saponin based buffer (BD perm/wash)
    Gating Strategy
    Singlets were excluded. Alive cells were selected according to the exclusion of the viability dye. CD8+ cells were gated for the analysis of 11bHSD1.
    Specification
    murine T cells from spleen
    Preparation
    Cell harvesting/tissue preparation method: Spleen was harvested and pass through a 100 um nylon strainer. Red blood cell lysis was performed. Extracellular staining was first performed, followed by fixation and permeabilization previous the intracellular staining.
    Sample buffer: KDS-BSS
    Fixation
    Paraformaldehyde
    Username

    Dr. Lourdes Rocamora-Reverte

    Verified customer

    Submitted Jul 16 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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