Product nameAnti-HSD11B1 antibody [EP9406(2)]
See all HSD11B1 primary antibodies
DescriptionRabbit monoclonal [EP9406(2)] to HSD11B1
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Guinea pig, Hamster
Synthetic peptide, corresponding to residues in Human HSD11B1 (Uniprot ID: P28845)
- Recombinant Human HSD11B1 protein (ab114301) can be used as a positive control in WB. Human fetal liver lysate. Raji lysate. Human kidney and liver tissues.
Mouse and rat species are recommended based on WB results, we do not guarantee IHC-P for mouse and rat.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab169785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 32 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
We do not guarantee IHC-P for mouse and rat.
FunctionCatalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol.
Tissue specificityWidely expressed. Highest expression in liver.
Involvement in diseaseDefects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- 11 beta HSD 1 antibody
- 11 beta HSD1 antibody
- 11 beta hydroxysteroid dehydrogenase 1 antibody
All lanes : Anti-HSD11B1 antibody [EP9406(2)] (ab169785) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Raji lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 32 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling HSD11B1 using ab169785 at a 1/100 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling HSD11B1 using ab169785 at a 1/100 dilution.
This product has been referenced in:
- Li Z et al. Hormonal changes and folliculogenesis in female offspring of rats exposed to cadmium during gestation and lactation. Environ Pollut 238:336-347 (2018). WB ; Rat . Read more (PubMed: 29574358) »
- Rocamora-Reverte L et al. T-cell autonomous death induced by regeneration of inert glucocorticoid metabolites. Cell Death Dis 8:e2948 (2017). Flow Cyt ; Mouse . Read more (PubMed: 28726773) »