Recombinant
RabMAb

Recombinant Anti-HSD11B1 antibody [EP9406(2)] - BSA and Azide free (ab240157)

Overview

  • Product name

    Anti-HSD11B1 antibody [EP9406(2)] - BSA and Azide free
    See all HSD11B1 primary antibodies
  • Description

    Rabbit monoclonal [EP9406(2)] to HSD11B1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Guinea pig, Hamster
  • Immunogen

    Synthetic peptide within Human HSD11B1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.

  • General notes

    ab240157 is the carrier-free version of ab169785 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240157 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse and rat species are recommended based on WB results, we do not guarantee IHC-P for mouse and rat.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

We do not guarantee IHC-P for mouse and rat.

 

WB Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol.
    • Tissue specificity

      Widely expressed. Highest expression in liver.
    • Involvement in disease

      Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
    • Sequence similarities

      Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    • Post-translational
      modifications

      Glycosylated.
    • Cellular localization

      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 11 beta HSD 1 antibody
      • 11 beta HSD1 antibody
      • 11 beta hydroxysteroid dehydrogenase 1 antibody
      • 11 DH antibody
      • 11-beta hydroxysteroid dehydrogenase, type 1 antibody
      • 11-beta-HSD1 antibody
      • 11-beta-hydroxysteroid dehydrogenase 1 antibody
      • 11-DH antibody
      • 11DH antibody
      • Corticosteroid 11 beta dehydrogenase isozyme 1 antibody
      • Corticosteroid 11-beta-dehydrogenase isozyme 1 antibody
      • CORTRD2 antibody
      • DHI1_HUMAN antibody
      • HDL antibody
      • HSD 11 antibody
      • HSD11 antibody
      • HSD11B antibody
      • HSD11B1 antibody
      • HSD11L antibody
      • Hydroxysteroid (11 beta) dehydrogenase antibody
      • Hydroxysteroid (11 beta) dehydrogenase 1 antibody
      • MGC13539 antibody
      • SDR26C1 antibody
      • short chain dehydrogenase/reductase family 26C, member 1 antibody
      see all

    Images

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling HSD11B1 using ab169785 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169785).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling HSD11B1 using ab169785 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169785).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab240157 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab240157.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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