RecombinantRabMAb

Recombinant Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)

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Flow Cytometry - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)
  • Immunocytochemistry - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)
  • Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9407(2)] to HSD11B1
  • Suitable for: ICC, Flow Cyt, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-HSD11B1 antibody [EPR9407(2)]
    See all HSD11B1 primary antibodies

  • Description

    Rabbit monoclonal [EPR9407(2)] to HSD11B1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to a region within Human HSD11B1 (P28845).

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab157223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
ICC
1/50 - 1/100.
Flow Cyt
1/30.
WB
1/10000 - 1/50000. Predicted molecular weight: 32 kDa.
IHC-P
1/1500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC
1/50 - 1/100.
Flow Cyt
1/30.
WB
1/10000 - 1/50000. Predicted molecular weight: 32 kDa.
IHC-P
1/1500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol.

  • Tissue specificity

    Widely expressed. Highest expression in liver.

  • Involvement in disease

    Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).

  • Sequence similarities

    Belongs to the short-chain dehydrogenases/reductases (SDR) family.

  • Post-translational
    modifications

    Glycosylated.

  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • 11 beta HSD 1 antibody
      • 11 beta HSD1 antibody
      • 11 beta hydroxysteroid dehydrogenase 1 antibody
      • 11 DH antibody
      • 11-beta hydroxysteroid dehydrogenase, type 1 antibody
      • 11-beta-HSD1 antibody
      • 11-beta-hydroxysteroid dehydrogenase 1 antibody
      • 11-DH antibody
      • 11DH antibody
      • Corticosteroid 11 beta dehydrogenase isozyme 1 antibody
      • Corticosteroid 11-beta-dehydrogenase isozyme 1 antibody
      • CORTRD2 antibody
      • DHI1_HUMAN antibody
      • HDL antibody
      • HSD 11 antibody
      • HSD11 antibody
      • HSD11B antibody
      • HSD11B1 antibody
      • HSD11L antibody
      • Hydroxysteroid (11 beta) dehydrogenase antibody
      • Hydroxysteroid (11 beta) dehydrogenase 1 antibody
      • MGC13539 antibody
      • SDR26C1 antibody
      • short chain dehydrogenase/reductase family 26C, member 1 antibody
      see all

    Images

    • Flow Cytometry - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)
      Flow Cytometry - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)
      Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling HSD11B1 with Purified ab157223 at 1:30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor ® 488 ,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    • Immunocytochemistry - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)
      Immunocytochemistry - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)
      Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling HSD11B1 with Purified ab157223 at 1:50 dilution (5.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)
      Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)

    References (0)

    Publishing research using ab157223? Please let us know so that we can cite the reference in this datasheet.

    ab157223 has not yet been referenced specifically in any publications.

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