Recombinant
RabMAb

Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)

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Overview

  • Product name
    Anti-HSD11B1 antibody [EPR9407(2)]
    See all HSD11B1 primary antibodies
  • Description
    Rabbit monoclonal [EPR9407(2)] to HSD11B1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human HSD11B1 (P28845).

  • Positive control
    • Human fetal liver lysate; Human hepatocellular carcinoma tissue, Human ovarian carcinoma tissue; HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab157223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 32 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function
      Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol.
    • Tissue specificity
      Widely expressed. Highest expression in liver.
    • Involvement in disease
      Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
    • Sequence similarities
      Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    • Post-translational
      modifications
      Glycosylated.
    • Cellular localization
      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
      • Alternative names
        • 11 beta HSD 1 antibody
        • 11 beta HSD1 antibody
        • 11 beta hydroxysteroid dehydrogenase 1 antibody
        • 11 DH antibody
        • 11-beta hydroxysteroid dehydrogenase, type 1 antibody
        • 11-beta-HSD1 antibody
        • 11-beta-hydroxysteroid dehydrogenase 1 antibody
        • 11-DH antibody
        • 11DH antibody
        • Corticosteroid 11 beta dehydrogenase isozyme 1 antibody
        • Corticosteroid 11-beta-dehydrogenase isozyme 1 antibody
        • CORTRD2 antibody
        • DHI1_HUMAN antibody
        • HDL antibody
        • HSD 11 antibody
        • HSD11 antibody
        • HSD11B antibody
        • HSD11B1 antibody
        • HSD11L antibody
        • Hydroxysteroid (11 beta) dehydrogenase antibody
        • Hydroxysteroid (11 beta) dehydrogenase 1 antibody
        • MGC13539 antibody
        • SDR26C1 antibody
        • short chain dehydrogenase/reductase family 26C, member 1 antibody
        see all

      Images

      • Western blot - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)
        Western blot - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)
        Anti-HSD11B1 antibody [EPR9407(2)] (ab157223) at 1/10000 dilution + Human fetal liver lysate at 10 µg

        Secondary
        Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

        Predicted band size: 32 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)
        Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labeling HSD11B with ab157223 at 1/50.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)
        Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling HSD11B with ab157223 at 1/50.
      • Immunocytochemistry/ Immunofluorescence - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)
        Immunocytochemistry/ Immunofluorescence - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)
        Immunofluorescent analysis of HepG2 cells labeling HSD11B1 with ab157223 at 1/50.

      References

      ab157223 has not yet been referenced specifically in any publications.

      Publishing research using ab157223? Please let us know so that we can cite the reference in this datasheet.

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