Product nameAnti-HSD11B1 antibody [EPR9407(2)]
See all HSD11B1 primary antibodies
DescriptionRabbit monoclonal [EPR9407(2)] to HSD11B1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to a region within Human HSD11B1 (P28845).
- Human fetal liver lysate; Human hepatocellular carcinoma tissue, Human ovarian carcinoma tissue; HepG2 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab157223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 32 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/50 - 1/100.|
FunctionCatalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol.
Tissue specificityWidely expressed. Highest expression in liver.
Involvement in diseaseDefects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- 11 beta HSD 1 antibody
- 11 beta HSD1 antibody
- 11 beta hydroxysteroid dehydrogenase 1 antibody
Anti-HSD11B1 antibody [EPR9407(2)] (ab157223) at 1/10000 dilution + Human fetal liver lysate at 10 µg
Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 32 kDa
Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labeling HSD11B with ab157223 at 1/50.
Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling HSD11B with ab157223 at 1/50.
Immunofluorescent analysis of HepG2 cells labeling HSD11B1 with ab157223 at 1/50.
ab157223 has not yet been referenced specifically in any publications.