Recombinant Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-HSD11B1 antibody [EPR9407(2)]
See all HSD11B1 primary antibodies -
Description
Rabbit monoclonal [EPR9407(2)] to HSD11B1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a region within Human HSD11B1 (P28845).
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Positive control
- Human fetal liver lysate; Human hepatocellular carcinoma tissue, Human ovarian carcinoma tissue; HepG2 cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9407(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab157223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 32 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/50 - 1/100. |
Target
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Function
Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol. -
Tissue specificity
Widely expressed. Highest expression in liver. -
Involvement in disease
Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS). -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 3290 Human
- Omim: 600713 Human
- SwissProt: P28845 Human
- Unigene: 195040 Human
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Alternative names
- 11 beta HSD 1 antibody
- 11 beta HSD1 antibody
- 11 beta hydroxysteroid dehydrogenase 1 antibody
see all
Images
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![Western blot - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)](https://www.abcam.com/ps/products/157/ab157223/Images/ab157223-164442-anti-hsd11b1-antibody-epr94072-western-blot.jpg)
Western blot - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)Anti-HSD11B1 antibody [EPR9407(2)] (ab157223) at 1/10000 dilution + Human fetal liver lysate at 10 µg
Secondary
Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 32 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)](https://www.abcam.com/ps/products/157/ab157223/Images/ab157223-164441-anti-hsd11b1-antibody-epr94072-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labeling HSD11B with ab157223 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)](https://www.abcam.com/ps/products/157/ab157223/Images/ab157223-164440-anti-hsd11b1-antibody-epr94072-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling HSD11B with ab157223 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)](https://www.abcam.com/ps/products/157/ab157223/Images/ab157223-164439-anti-hsd11b1-antibody-epr94072-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)Immunofluorescent analysis of HepG2 cells labeling HSD11B1 with ab157223 at 1/50.
Protocols
Datasheets and documents
References
ab157223 has not yet been referenced specifically in any publications.
