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RecombinantRabMAb

Recombinant Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (ab175049)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (ab175049)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (ab175049)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (ab175049)
  • Immunocytochemistry - Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (ab175049)
  • Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (ab175049)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9407(2)] to HSD11B1 - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Human

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Recombinant Human HSD11B1 protein (ab114301)
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Overview

  • Product name

    Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free
    See all HSD11B1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9407(2)] to HSD11B1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HSD11B1. The exact sequence is proprietary.
    Database link: P28845

  • General notes

    ab175049 is the carrier-free version of ab157223 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab175049 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9407(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis

Associated products

  • Alternative Versions

    • Anti-HSD11B1 antibody [EPR9407(2)] (ab157223)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Recombinant Protein

    • Recombinant Human HSD11B1 protein (ab114301)
  • Related Products

    • Recombinant Human HSD11B1 protein (ab114301)

Applications

Our Abpromise guarantee covers the use of ab175049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol.
    • Tissue specificity

      Widely expressed. Highest expression in liver.
    • Involvement in disease

      Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
    • Sequence similarities

      Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    • Post-translational
      modifications

      Glycosylated.
    • Cellular localization

      Endoplasmic reticulum membrane.
    • Target information above from: UniProt accession P28845 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3290 Human
      • Omim: 600713 Human
      • SwissProt: P28845 Human
      • Unigene: 195040 Human
      • Alternative names

        • 11 beta HSD 1 antibody
        • 11 beta HSD1 antibody
        • 11 beta hydroxysteroid dehydrogenase 1 antibody
        • 11 DH antibody
        • 11-beta hydroxysteroid dehydrogenase, type 1 antibody
        • 11-beta-HSD1 antibody
        • 11-beta-hydroxysteroid dehydrogenase 1 antibody
        • 11-DH antibody
        • 11DH antibody
        • Corticosteroid 11 beta dehydrogenase isozyme 1 antibody
        • Corticosteroid 11-beta-dehydrogenase isozyme 1 antibody
        • CORTRD2 antibody
        • DHI1_HUMAN antibody
        • HDL antibody
        • HSD 11 antibody
        • HSD11 antibody
        • HSD11B antibody
        • HSD11B1 antibody
        • HSD11L antibody
        • Hydroxysteroid (11 beta) dehydrogenase antibody
        • Hydroxysteroid (11 beta) dehydrogenase 1 antibody
        • MGC13539 antibody
        • SDR26C1 antibody
        • short chain dehydrogenase/reductase family 26C, member 1 antibody
        see all

      Images

      • Western blot - Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (ab175049)
        Western blot - Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (ab175049)
        Anti-HSD11B1 antibody [EPR9407(2)] (ab157223) at 1/10000 dilution + Human fetal liver tissue lysate at 10 µg

        Secondary
        Goat anti-rabbit HRP conjugated at 1/2000 dilution

        Predicted band size: 32 kDa



        This data was developed using ab157223, the same antibody clone in a different buffer formulation.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (ab175049)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (ab175049)
        This data was developed using ab157223, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labeling HSD11B with ab157223 at 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (ab175049)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (ab175049)
        This data was developed using ab157223, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling HSD11B with ab157223 at 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunocytochemistry - Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (ab175049)
        Immunocytochemistry - Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (ab175049)
        This data was developed using ab157223, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells labeling HSD11B1 with ab157223 at 1/50.
      • Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (ab175049)
        Anti-HSD11B1 antibody [EPR9407(2)] - BSA and Azide free (ab175049)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab175049? Please let us know so that we can cite the reference in this datasheet.

      ab175049 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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