Key features and details
- Rabbit polyclonal to HSD11B2
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HSD11B2 antibody
See all HSD11B2 primary antibodies
DescriptionRabbit polyclonal to HSD11B2
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human kidney, lymph node and rectum tissue. ICC/IF: RT4 cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionCatalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.
Tissue specificityFound in placenta, kidney, pancreas, prostate, ovary, small intestine and colon.
Involvement in diseaseDefects in HSD11B2 are the cause of apparent mineralocorticoid excess (AME) [MIM:218030]. AME is a potentially fatal disease characterized by severe juvenile low-renin hypertension, sodium retention, hypokalemia and low levels of aldosterone. It often leads to nephrocalcinosis.
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
Cellular localizationMicrosome. Endoplasmic reticulum.
- Information by UniProt
- 11 beta HSD2 antibody
- 11 beta hydroxysteroid dehydrogenase type 2 antibody
- 11 DH2 antibody
Paraffin-embedded human rectum tissue stained for HSD11B2 using ab244510 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for HSD11B2 using ab244510 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human lymph node tissue stained for HSD11B2 using ab244510 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for HSD11B2 using ab244510 at 1/500 dilution in immunohistochemical analysis. Low expression, as expected.
PFA-fixed, Triton X-100 permeabilized RT4 (human urinary bladder cancer cell line) cells stained for HSD11B2 (green) using ab244510 at 4 μg/ml in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244510 has not yet been referenced specifically in any publications.