Western blot abreview for Anti-HSD11B2 antibody

Good
Abreviews
Application
Western blot
Loading amount
8 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% Tris/Glycine-SDS)
Sample
Sheep Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Goat Anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Detection

Detection method
ECL
Bands
Specific: 44, 50 kDa
Exposure
15 second(s)

Additional data

Additional Notes
All experimental work completed by Megan A. Walton...
Abcam response
Any feedback provided by our customers is much appreciated. For users wishing to test our products in untested species or applications, we would encourage them to participate in our Abtrial program, by which you will beneficiate from a discount off your next purchase for the value of the product tested by submitting your results to our website. The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

Dr. Rob Cordery-Cotter

Verified customer

Submitted Nov 18 2013

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