Western blot abreview for Anti-HSD11B2 antibody

Inconclusive
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Prostate Cell Lines)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
20 µg
Specification
Prostate Cell Lines
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey Blocking Buffer + 0.2% Tween
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye 800CW Goat (polyclonal) anti-Rabbit IgG (H
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Infrared
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 44 kDa Non-specific: 24, 76, 102 kDa

Additional data

Additional Notes
Picture shows Full Range Rainbow Marker (Amersham) on the left and prostate cell line lysate on the right.
Abcam response
Our Scientific Support team has investigated this negative Abreview, and we are resolving the issue in accordance with our Abpromise.

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Verified customer

Submitted Sep 29 2015

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