Western blot abreview for Anti-HSD11B2 antibody

Average
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
15 µg
Treatment
Feeding high fat for 8 weeks.
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.5%TBST + HSD2
Dilution
1/1000

Secondary antibody

Dilution
1/3000
Name
Non-Abcam antibody was used: AP132P | Goat Anti-Rabbit IgG Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 44 kDa

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Submitted Oct 24 2018

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