Product nameAnti-HSD17B1 antibody [EP1682Y]
See all HSD17B1 primary antibodies
DescriptionRabbit monoclonal [EP1682Y] to HSD17B1
Tested applicationsSuitable for: WB, Flow Cyt, IP, IHC-Pmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Human
Synthetic peptide within Human HSD17B1 (C terminal). The exact sequence is proprietary.
- WB: Human placenta lysate. IHC-P: Human placenta tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab51045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).|
|Flow Cyt||Use 1µg for 106 cells.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionFavors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH.
PathwaySteroid biosynthesis; estrogen biosynthesis.
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
- Information by UniProt
- 17 beta HSD 1 antibody
- 17 beta hydroxysteroid dehydrogenase type 1 antibody
- 17-beta-HSD 1 antibody
Anti-HSD17B1 antibody [EP1682Y] (ab51045) at 1/100000 dilution + Human placenta lysate at 10 µg
Goat anti-Rabbit HRP labeled. at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
Ab51045 (1/100 dilution) staining human HSD17B1 in paraffin embedded human placenta tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Overlay histogram showing JEG3 cells stained with ab51045 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51045, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
This product has been referenced in:
- Li F et al. siRNA-based breast cancer therapy by suppressing 17ß-hydroxysteroid dehydrogenase type 1 in an optimized xenograft cell and molecular biology model in vivo. Drug Des Devel Ther 13:757-766 (2019). Read more (PubMed: 30863015) »
- Konings GF et al. Blocking 17ß-hydroxysteroid dehydrogenase type 1 in endometrial cancer: a potential novel endocrine therapeutic approach. J Pathol 244:203-214 (2018). Read more (PubMed: 29144553) »