Overview

  • Product name

    Anti-HSD17B1 antibody [EP1682Y]
    See all HSD17B1 primary antibodies
  • Description

    Rabbit monoclonal [EP1682Y] to HSD17B1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IP, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HSD17B1 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Human placenta lysate. IHC-P: Human placenta tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab51045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
Flow Cyt Use 1µg for 106 cells.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH.
    • Pathway

      Steroid biosynthesis; estrogen biosynthesis.
    • Sequence similarities

      Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • 17 beta HSD 1 antibody
      • 17 beta hydroxysteroid dehydrogenase type 1 antibody
      • 17-beta-HSD 1 antibody
      • 17-beta-hydroxysteroid dehydrogenase type 1 antibody
      • 20 alpha-hydroxysteroid dehydrogenase antibody
      • 20-alpha-HSD antibody
      • DHB1_HUMAN antibody
      • E17KSR antibody
      • E2DH antibody
      • EDH17B1 antibody
      • EDH17B2 antibody
      • EDHB17 antibody
      • Estradiol 17 beta dehydrogenase 1 antibody
      • Estradiol 17-beta-dehydrogenase 1 antibody
      • HSD17 antibody
      • HSD17B1 antibody
      • Hydroxysteroid (17 beta) dehydrogenase 1 antibody
      • MGC13814 antibody
      • Placental 17 beta hydroxysteroid dehydrogenase antibody
      • Placental 17-beta-hydroxysteroid dehydrogenase antibody
      • SDR28C1 antibody
      • Short chain dehydrogenase/reductase family 28CE,member 1 antibody
      see all

    Images

    • Anti-HSD17B1 antibody [EP1682Y] (ab51045) at 1/100000 dilution + Human placenta lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled. at 1/2000 dilution

      Predicted band size: 35 kDa
      Observed band size: 35 kDa

    • Ab51045 (1/100 dilution) staining human HSD17B1 in paraffin embedded human placenta tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Overlay histogram showing JEG3 cells stained with ab51045 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51045, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

    References

    This product has been referenced in:

    • Li F  et al. siRNA-based breast cancer therapy by suppressing 17ß-hydroxysteroid dehydrogenase type 1 in an optimized xenograft cell and molecular biology model in vivo. Drug Des Devel Ther 13:757-766 (2019). Read more (PubMed: 30863015) »
    • Konings GF  et al. Blocking 17ß-hydroxysteroid dehydrogenase type 1 in endometrial cancer: a potential novel endocrine therapeutic approach. J Pathol 244:203-214 (2018). Read more (PubMed: 29144553) »
    See all 10 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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