Anti-HSD17B1 antibody [EP1682Y] (ab51045)
- Datasheet
- References (8)
- Protocols
Overview
-
Product nameAnti-HSD17B1 antibody [EP1682Y]
See all HSD17B1 primary antibodies -
DescriptionRabbit monoclonal [EP1682Y] to HSD17B1
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, ICC/IF, Flow Cyt, IP, IHC-Pmore details
-
Species reactivityReacts with: Human
-
Immunogen
Synthetic peptide within Human HSD17B1 (C terminal). The exact sequence is proprietary.
-
Positive control
- WB: Human placenta lysate. IHC-P: Human placenta tissue.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEP1682Y
-
IsotypeIgG
-
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab51045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). | |
| ICC/IF | 1/100 - 1/250. | |
| Flow Cyt | Use 1µg for 106 cells. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IP | 1/100. | |
| IHC-P | 1/100 - 1/250. |
Target
-
FunctionFavors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH.
-
PathwaySteroid biosynthesis; estrogen biosynthesis.
-
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
-
Cellular localizationCytoplasm.
- Information by UniProt
-
Database links
- Entrez Gene: 3292 Human
- Omim: 109684 Human
- SwissProt: P14061 Human
- Unigene: 654385 Human
- Unigene: 655222 Human
-
Alternative names
- 17 beta HSD 1 antibody
- 17 beta hydroxysteroid dehydrogenase type 1 antibody
- 17-beta-HSD 1 antibody
see all
Images
-
![Western blot - Anti-HSD17B1 antibody [EP1682Y] (ab51045)](https://www.abcam.com/ps/products/51/ab51045/Images/ab51045_1.jpg)
Western blot - Anti-HSD17B1 antibody [EP1682Y] (ab51045)Anti-HSD17B1 antibody [EP1682Y] (ab51045) at 1/100000 dilution + Human placenta lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled. at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B1 antibody [EP1682Y] (ab51045)](https://www.abcam.com/ps/products/51/ab51045/Images/ab51045_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B1 antibody [EP1682Y] (ab51045)Ab51045 (1/100 dilution) staining human HSD17B1 in paraffin embedded human placenta tissue by Immunohistochemistry. -
![Flow Cytometry - Anti-HSD17B1 antibody [EP1682Y] (ab51045)](https://www.abcam.com/ps/products/51/ab51045/Images/HSD17B1-Primary-antibodies-ab51045-1.jpg)
Flow Cytometry - Anti-HSD17B1 antibody [EP1682Y] (ab51045)Overlay histogram showing JEG3 cells stained with ab51045 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51045, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
Datasheets and documents
References
This product has been referenced in:
- Konings GF et al. Blocking 17ß-hydroxysteroid dehydrogenase type 1 in endometrial cancer: a potential novel endocrine therapeutic approach. J Pathol 244:203-214 (2018). Read more (PubMed: 29144553) »
- Sinreih M et al. The Significance of the Sulfatase Pathway for Local Estrogen Formation in Endometrial Cancer. Front Pharmacol 8:368 (2017). Read more (PubMed: 28690541) »
