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RecombinantRabMAb

Recombinant Anti-HSD17B1 antibody [EP1682Y] (ab51045)

  • Datasheet
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Western blot - Anti-HSD17B1 antibody [EP1682Y] (ab51045)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B1 antibody [EP1682Y] (ab51045)
  • Immunoprecipitation - Anti-HSD17B1 antibody [EP1682Y] (ab51045)
  • Flow Cytometry (Intracellular) - Anti-HSD17B1 antibody [EP1682Y] (ab51045)
  • Anti-HSD17B1 antibody [EP1682Y] (ab51045)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1682Y] to HSD17B1
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Recombinant Human HSD17B1 protein (ab156332)

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Overview

  • Product name

    Anti-HSD17B1 antibody [EP1682Y]
    See all HSD17B1 primary antibodies
  • Description

    Rabbit monoclonal [EP1682Y] to HSD17B1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HSD17B1 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Human placenta lysate. IHC-P: Human placenta tissue. IP: Human placenta lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1682Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Neuroscience
    • Endocrine system
    • Gonadotrophic axis
    • Cancer
    • Growth factors
    • Hormones
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free (ab247308)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human HSD17B1 protein (ab156332)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab51045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use 1µg for 106 cells.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/10000 - 1/50000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IP
1/50.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
Use 1µg for 106 cells.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/10000 - 1/50000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IP
1/50.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for ICC/IF.

Target

  • Function

    Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH.
  • Pathway

    Steroid biosynthesis; estrogen biosynthesis.
  • Sequence similarities

    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P14061 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3292 Human
    • Omim: 109684 Human
    • SwissProt: P14061 Human
    • Unigene: 654385 Human
    • Unigene: 655222 Human
    • Alternative names

      • 17 beta HSD 1 antibody
      • 17 beta hydroxysteroid dehydrogenase type 1 antibody
      • 17-beta-HSD 1 antibody
      • 17-beta-hydroxysteroid dehydrogenase type 1 antibody
      • 20 alpha-hydroxysteroid dehydrogenase antibody
      • 20-alpha-HSD antibody
      • DHB1_HUMAN antibody
      • E17KSR antibody
      • E2DH antibody
      • EDH17B1 antibody
      • EDH17B2 antibody
      • EDHB17 antibody
      • Estradiol 17 beta dehydrogenase 1 antibody
      • Estradiol 17-beta-dehydrogenase 1 antibody
      • HSD17 antibody
      • HSD17B1 antibody
      • Hydroxysteroid (17 beta) dehydrogenase 1 antibody
      • MGC13814 antibody
      • Placental 17 beta hydroxysteroid dehydrogenase antibody
      • Placental 17-beta-hydroxysteroid dehydrogenase antibody
      • SDR28C1 antibody
      • Short chain dehydrogenase/reductase family 28CE,member 1 antibody
      see all

    Images

    • Western blot - Anti-HSD17B1 antibody [EP1682Y] (ab51045)
      Western blot - Anti-HSD17B1 antibody [EP1682Y] (ab51045)
      Anti-HSD17B1 antibody [EP1682Y] (ab51045) at 1/100000 dilution + Human placenta lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled. at 1/2000 dilution

      Predicted band size: 35 kDa
      Observed band size: 35 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B1 antibody [EP1682Y] (ab51045)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B1 antibody [EP1682Y] (ab51045)

      Ab51045 (1/100 dilution) staining human HSD17B1 in paraffin embedded human placenta tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-HSD17B1 antibody [EP1682Y] (ab51045)
      Immunoprecipitation - Anti-HSD17B1 antibody [EP1682Y] (ab51045)

      Purified ab51045 at 1/50 dilution (2µg) immunoprecipitating HSD17B1 in Human placenta lysate.
      Lane 1 (input): Human placenta lysate 10µg
      Lane 2 (+): ab51045 + Human placenta lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51045 in Human placenta lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 34 kDa

    • Flow Cytometry (Intracellular) - Anti-HSD17B1 antibody [EP1682Y] (ab51045)
      Flow Cytometry (Intracellular) - Anti-HSD17B1 antibody [EP1682Y] (ab51045)

      Overlay histogram showing JEG3 cells stained with ab51045 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51045, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

       

    • Anti-HSD17B1 antibody [EP1682Y] (ab51045)
      Anti-HSD17B1 antibody [EP1682Y] (ab51045)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (14)

    Publishing research using ab51045? Please let us know so that we can cite the reference in this datasheet.

    ab51045 has been referenced in 14 publications.

    • Nedder M  et al. Uptake of Cerium Dioxide Nanoparticles and Impact on Viability, Differentiation and Functions of Primary Trophoblast Cells from Human Placenta. Nanomaterials (Basel) 10:N/A (2020). PubMed: 32635405
    • Huang ZX  et al. Establishment of an immortalized stromal cell line derived from human Endometriotic lesion. Reprod Biol Endocrinol 18:119 (2020). PubMed: 33225937
    • Zou Z  et al. Potential role of microRNA-424 in regulating ERR? to suppress trophoblast proliferation and invasion in fetal growth restriction. Placenta 83:57-62 (2019). PubMed: 31477209
    • Li F  et al. siRNA-based breast cancer therapy by suppressing 17ß-hydroxysteroid dehydrogenase type 1 in an optimized xenograft cell and molecular biology model in vivo. Drug Des Devel Ther 13:757-766 (2019). PubMed: 30863015
    • Konings GF  et al. Blocking 17ß-hydroxysteroid dehydrogenase type 1 in endometrial cancer: a potential novel endocrine therapeutic approach. J Pathol 244:203-214 (2018). PubMed: 29144553
    View all Publications for this product

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