Overview

  • Product name

  • Description

    Rabbit polyclonal to Hsd17b11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human Hsd17b11 aa 58-107. The exact sequence is proprietary.

  • Positive control

    • RAW 264.7 cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS is without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab136109 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 33 kDa.

Target

  • Function

    Can convert androstan-3-alpha,17-beta-diol (3-alpha-diol) to androsterone in vitro, suggesting that it may participate in androgen metabolism during steroidogenesis. May act by metabolizing compounds that stimulate steroid synthesis and/or by generating metabolites that inhibit it. Has no activity toward DHEA (dehydroepiandrosterone), or A-dione (4-androste-3,17-dione), and only a slight activity toward testosterone to A-dione. Tumor-associated antigen in cutaneous T-cell lymphoma.
  • Tissue specificity

    Present at high level in steroidogenic cells such as syncytiotrophoblasts, sebaceous gland, Leydig cells, and granulosa cells of the dominant follicle and corpus luteum. In lung, it is detected in the ciliated epithelium and in acini of adult trachea, in bronchioles, but not in alveoli. In the eye, it is detected in the nonpigmented epithelium of the ciliary body and, at lower level, in the inner nuclear layer of the retina (at protein level). Widely expressed. Highly expressed in retina, pancreas, kidney, liver, lung, adrenal, small intestine, ovary and heart.
  • Sequence similarities

    Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • 17 beta HSD 11 antibody
    • 17 beta HSD XI antibody
    • 17 BETA HSD11 antibody
    • 17 BETA HSDXI antibody
    • 17 beta hydroxysteroid dehydrogenase 11 antibody
    • 17 beta hydroxysteroid dehydrogenase type XI antibody
    • 17 beta hydroxysteroid dehydrogenase XI antibody
    • 17-beta-HSD 11 antibody
    • 17-beta-HSD XI antibody
    • 17-beta-hydroxysteroid dehydrogenase 11 antibody
    • 17-beta-hydroxysteroid dehydrogenase XI antibody
    • 17betaHSD11 antibody
    • 17betaHSDXI antibody
    • 17bHSD11 antibody
    • CTCL associated antigen HD CL 03 antibody
    • CTCL tumor antigen HD CL 03 antibody
    • CTCL-associated antigen HD-CL-03 antibody
    • Cutaneous T cell lymphoma associated antigen HD CL 03 antibody
    • Cutaneous T-cell lymphoma-associated antigen HD-CL-03 antibody
    • Dehydrogenase/reductase SDR family member 8 antibody
    • DHB11_HUMAN antibody
    • DHRS8 antibody
    • Estradiol 17 beta dehydrogenase 11 antibody
    • Estradiol 17-beta-dehydrogenase 11 antibody
    • Hsd17b11 antibody
    • Hydroxysteroid (17 beta) dehydrogenase 11 antibody
    • PAN1B antibody
    • Retinal short chain dehydrogenase/reductase 2 antibody
    • Retinal short-chain dehydrogenase/reductase 2 antibody
    • RETSDR2 antibody
    • SDR16C2 antibody
    • SDR2 antibody
    • Short chain dehydrogenase/reductase family 16C member 2 antibody
    • T cell lymphoma associated antigen HD CL 03 antibody
    see all

Images

  • Anti-Hsd17b11 antibody (ab136109) at 1/500 dilution + RAW 264.7 cell lysate at 30 µg

    Predicted band size: 33 kDa

References

This product has been referenced in:

  • Liu Y  et al. Hydroxysteroid dehydrogenase family proteins on lipid droplets through bacteria, C. elegans, and mammals. Biochim Biophys Acta 1863:881-894 (2018). WB . Read more (PubMed: 29702244) »
  • Deng R  et al. Identification and characterization of ERV transcripts in goat embryos. Reproduction N/A:N/A (2018). Read more (PubMed: 30407918) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Lipid droplet protein precipitated)
Gel Running Conditions
Reduced Denaturing (4-12% Bio-rad gel)
Loading amount
15 µg
Treatment
adeno over expression
Specification
Lipid droplet protein precipitated
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 07 2017

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