Key features and details
- Rabbit polyclonal to HSD17B13
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-HSD17B13 antibody
DescriptionRabbit polyclonal to HSD17B13
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
- IHC: Human liver and skin tissue sections; ICC/IF: Human cell line U-251MG.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificityHighly expressed in the liver. Also detected in ovary, bone marrow, kidney, brain, lung, skeletal muscle, bladder and testis.
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
- Information by UniProt
- 17-beta-HSD 13 antibody
- 17-beta-hydroxysteroid dehydrogenase 13 antibody
- DHB13_HUMAN antibody
Immunohistochemical analysis of human liver tissue labeling HSD17B13 with ab122036 at 1/200 dilution. Moderate to strong granular cytoplasmic positivity in hepatocytes is shown.
Immunofluorescent staining of Human cell line U-251MG shows positivity in golgi apparatus and vesicles. Recommended concentration of ab122036 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemical analysis of human skin tissue labeling HSD17B13 with ab122036 at 1/200 dilution. Moderate granular cytoplasmic positivity in basal epidermal cells is shown.
Immunohistochemical analysis of human tonsil tissue labeling HSD17B13 with ab122036 at 1/200 dilution. As expected, no positivity in lymphoid cells is shown.
Immunohistochemical analysis of human placenta tissue labeling HSD17B13 with ab122036 at 1/500 dilution. As expected, no positivity in trophoblastic cells is shown.
ab122036 has been referenced in 2 publications.
- Su W et al. Liver X receptor a induces 17ß-hydroxysteroid dehydrogenase-13 expression through SREBP-1c. Am J Physiol Endocrinol Metab 312:E357-E367 (2017). PubMed: 28270440
- Su W et al. Comparative proteomic study reveals 17ß-HSD13 as a pathogenic protein in nonalcoholic fatty liver disease. Proc Natl Acad Sci U S A 111:11437-42 (2014). Human . PubMed: 25028495